GARY A. BANKER

Education:

1964 - 1966  Antioch College, Yellow Springs, OH

1966 - 1968  B.S. magna cum laude, Mathematics and Psychology, University of Washington, Seattle, WA

1968 - 1973  Ph.D., Psychobiology, University of California at Irvine, CA.
Advisor:  C.W. Cotman; Thesis:  Proteins of Synaptic Structures

1973 - 1976  Postdoctoral Fellow, Dept. of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, MO.  Advisor:  W.M. Cowan

Academic Appointments:

1998-present  Senior Scientist and Director of the Program in Cellular Neurobiology, Center for Research on Occupational and Environmental Toxicology and Professor of Cell and Developmental Biology, Oregon Health and Science University, Portland, Oregon

1997   John T. and Louise Nerancy Professor of Neurosciences, University of Virginia School of Medicine, Charlottesville, Virginia

1989-1997  Professor of Neuroscience, University of Virginia School of Medicine, Charlottesville, Virginia

1976 - 1989  Assistant, Associate, and Full Professor of Anatomy, Cell Biology, and Neurobiology, Albany Medical College, Albany, New York

1988 - 1989  Professor of Biomedical Sciences, Division of Neuroscience, School of Public Health, State University of New York at Albany, Albany, New York

1987 ‑1988  Visiting Professor, Section of Molecular Neurobiology, Yale University School of Medicine, New Haven, Connecticut

1985   Visiting Scientist, Laboratory of Developmental Neurobiology, Salk Institute, San Diego, CA

1976 - 1979  Adjunct Assistant Professor, Dept. of Physical Therapy, Russell Sage College, Troy, NY
Honors and Awards:

1964 - 1968  National Merit Scholar
1968   Phi Beta Kappa
1968   National Science Foundation, Undergraduate Trainee
1968 - 1973  National Institute of Mental Health, Predoctoral Trainee and Fellow
1974 - 1976  National Institutes of Health, Postdoctoral Fellow
1976 - 1980  Research Fellow of the Sinsheimer Foundation
1980   Harold C. Wiggers Travel Fellowship
1985   Schaffer Faculty Development Award
1989 - 1996  Javits Neuroscience Investigator Award (NIH)
1994   Dean's Award for Excellence in Teaching
1996   David Harrison Award for Outstanding Teaching
1998, 1999, 2000 Grass Foundation Traveling Lecturer

Professional Activities:

Director, Multidisciplinary Training Program in Neuroscience, Oregon Health and Science University (1999-present).

Executive Committee, Nanobiotechnology Center, Cornell University (2000-present).

Editorial Board:  Journal of Neurocytology (1990-present); European Journal of Neuroscience (1999-2003).

National Institutes of Health Study Sections and Review Committees: Synapses, Cytoskeleton And Trafficking Study Section (2003-present); Javits Centers Program (1985); Neurology A, B1 and B2, NST ad hoc (1985-present); Neurology B2, regular member (1993-1995); NICHHD:  Intramural Programs Review (1990, 1998).

Neurobiology Course, Marine Biological Laboratory, Woods Hole, MA:  instructor (1989-1999); head, section on structure and development (1991-1994); course director (1995-1999)

Memberships:  American Society for Cell Biology; Society for Neuroscience (Hudson-Berkshire Chapter President, 1983‑1985)

Current Grant Support:

National Institutes of Health (R01 NS 17112‑22):  The development of hippocampal neurons in culture (Principal Investigator); 2/1/04 to 1/31/09; current year direct costs: $231,000.

National Institutes of Health (R01 MH66179-02): Neuronal polarity and membrane trafficking.  (Principal Investigator); 7/1/02 to 6/30/07; current year direct costs: $225,000.

National Science Foundation Science and Technology Center (9876771): The Nanobiotechnology Center: Applications of nanofabrication to neurobiology (Co-investigator).  Subcontract from Cornell University;1/1/00 to 12/31/10; current year direct costs: $160,000.

National Institutes of Health (T32 NS07466-06): Multidisciplinary Training in Neuroscience (Principal Investigator); 7/1/99 to 6/30/09; current year direct costs: $150,298.

Publications--Books and Invited Chapters:

Cotman, C.W. and Banker, G.A. (1974)  The making of a synapse.  In: Reviews of Neuroscience, Ehrenpreis, S. and Kopin, I.J., (eds.), vol. 1, pp. 1‑61.

Steward, O., Davis, L., Reeves, T.M., and Banker, G. (1988)  Microcompartmentation of the protein synthetic machinery of neurons:  polyribosome localization under postsynaptic sites.  In:  Intracellullular Determinants of Neuronal Form and Function, (R. Lasek and M.B. Black, eds.), Alan R. Liss, New York, pps. 521‑544.

Banker, G.A., and Waxman, A.B. (1988) Hippocampal neurons generate natural shapes in cell culture.  In: Intracellullular Determinants of Neuronal Form and Function, (R. Lasek and M.B. Black, eds.), Alan R. Liss, New York, pps. 61‑82.

Steward, O., Banker, G.A., Davis, L., and Phillips, L.L. (1988)  The process of reinnervation of CNS neurons: evidence for local synthesis of constituents at postsynaptic sites.  In: Neural Degeneration and Regeneration (A. Gorio, J. deVellis, J.R. Perez-Polo, B. Haber, eds.), Springer-Verlag, Berlin, pps. 469‑480.

Steward, O., Davis, L., Dotti, C., Phillips, L., Rao, A., and Banker, G. (1988)  Protein synthesis and processing in cytoplasmic microdomains beneath postsynaptic sites on CNS neurons: A mechanism for establishing and maintaining a mosaic postsynaptic receptive surface.  Molec. Neurobiol.  2:227‑261.

Banker, G. and Goslin K. (1988)  Developments in neuronal cell culture.  Nature 336:185‑186.

Banker, G. and Goslin, K. (1991)  Culturing Nerve Cells.  Cambridge, MA:  MIT Press.

Vale, R.D., Banker, G. and Hall, Z.W. (1992)  The neuronal cytoskeleton.  In:  Molecular Neurobiology (Z.W. Hall, ed.), Sinauer Associates, Sunderland, MA., pps. 247‑280.

Steward, O., and Banker, G. (1992)  Getting the message from the gene to the synapse: sorting and intracellular transport of RNA in neurons and other spatially complex cells.  Trends Neurosci. 15:180‑186.

Craig, A.M., Jareb, M., and Banker, G. (1992) Neuronal Polarity.  Curr. Opinion Neurobiol. 2: 602-606.

Craig, A.M., and Banker, G. (1994)  Neuronal Polarity.  Annu. Rev. Neurosci. 17: 267-310.

Steward, O., Kleiman, R., and Banker, G. (1994) Sorting and Intracellular transport of RNA in neurons: regulation of gene expression at synaptic sites.  In: Regulation of gene expression and brain function (P.J. Harrison, ed.), Springer-Verlag, Berlin, pps 17-29.

Futerman, A.H. and Banker, G.A. (1996) The economics of neurite outgrowth--the addition of new membrane to growing axons.  Trends Neurosci. 19: 144-149.

Mandell, J.W., and Banker, G. (1996)  Microtubule-associated proteins, phosphorylation gradients, and the establishment of neuronal polarity.  Perspectives Develop. Neurobiol.  4:  125-135.

Higgins, D., Burack, M., Lein, P., and Banker, G. (1997)  Mechanisms of Neuronal Polarity. Curr. Opinion Neurobiol. 7: 599‑604.

Banker, G. and Goslin, K. (1998)  Culturing Nerve Cells (Second Edition).  Cambridge, MA: MIT Press.

Silverman, M. A., Kaech, S., Burack, M.A., and Banker, G. (2000) Vesicle transport in neurons.  In: GFP in Motion (ed. B. Ludin): a video supplement to Trends in Cell Biology.

Banker G.  (2003)  Pars, PI 3-kinase, and the establishment of neuronal polarity.
Cell 112: 4-5.

Original Articles:

Banker, G.A., Hunt, E., and Pagano, R. (1969) Evidence supporting the memory  disruption hypothesis of electro-convulsive shock action.  Physiol. and Behav. 4:895‑899.
 
Cotman, C.W., Banker, G.A., Zornetzer, S.F., and McGaugh,J.L. (1971)  Electro-shock effects on brain protein synthesis: Relation to brain seizures and retrograde amnesia.  Science 173:454‑456.

Banker, G.A. and Cotman, C.W. (1971) Characteristics of different amino acids as protein precursors in mouse brain:  Advantages of certain carboxyl-labeled amino acids.  Arch. Biochem. Biophys. 142:565‑573.

Cotman, C.W., Levy, W., Banker, G.A., and Taylor, D. (1971) An ultrastructural and chemical analysis of the effect of Triton X‑100 on synaptic plasma membrane.  Biochem. Biophys. Acta 249:406‑418.

Banker, G.A., Crain, B., and Cotman, C.W. (1972) Molecular weights of the polypeptide chains of synaptic plasma membranes.  Brain Res. 42:508‑513.

Banker, G.A. and Cotman, C.W. (1972) Measurement of free electrophoretic mobility and retardation coefficient of protein-sodium dodecyl sulfate complexes by gel electrophoresis:  A method to validate molecular weight estimates.  J. Biol. Chem. 247:5856‑5861.

Doyle, L.C., Banker, G.A., and Cotman, C.W. (1973) Gradient design to optimize rate zonal separations.  Anal. Biochem. 56:370‑382.
Cotman, C.W., Banker, G.A., Churchill, L., and Taylor, D. (1974) Isolation of  postsynaptic densities from rat brain.  J. Cell Biol. 63:441‑455.

Banker, G.A., Churchill, L., and Cotman, C.W. (1974) Proteins of the postsynaptic density.  J. Cell Biol. 63:456‑465.

Churchill, L., Cotman, C.W., Banker, G.A., Kelly, P., and Shannon, L. (1976)  Carbohydrate composition of central nervous system synapses:  Analysis of  isolated synaptic junctional complexes and postsynaptic densities.  Biochem.  Biophys. Acta. 448:57‑72.

Banker, G.A. and Cowan, W.M. (1977) Rat hippocampal neurons in dispersed cell culture.  Brain Res. 126:397‑425.

Banker, G.A. and Cowan, W.M. (1979) Some further observations on hippocampal neurons in dispersed cell culture.  J. Comp. Neurol. 187:469‑493.

Stieg, P.E., Kimelberg, H.K., Mazurkiewicz, J.E., and Banker, G.A. (1980)  Distribution of glial fibrillary acidic protein and fibronectin in primary astroglial cultures from rat brain.  Brain Res. 199:493‑500.

Banker, G.A. (1980) Trophic interactions between astroglia and hippocampal  neurons in culture.  Science 209:809‑810.

Bartlett, W.P and Banker, G.A. (1984) Electron microscopic studies of axonal and dendritic development by hippocampal neurons in culture.  I. Cells which develop without intercellular contacts.  J. Neurosci. 4:1944‑1953.

Bartlett, W.P. and Banker, G.A. (1984) Electron microscopic studies of axonal and dendritic development by hippocampal neurons in culture.  II. Synaptic relationships.  J. Neurosci. 4:1954‑1965.

Caceres, A., Banker, G.A., Steward, O., Binder, L. and Payne, M. (1984) MAP2 is localized to the dendrites of hippocampal neurons which develop in culture.  Devel. Brain Res. 13:314‑318.

Shaw, G., Banker G.A., and Weber, K. (1985) An immunofluorescence study of neurofilament protein expression by developing hippocampal neurons in tissue culture.  Europ. J. of Cell Biol. 39:205‑216.

Caceres, A., Banker, G.A., and Binder, L. (1986) Immunocytochemical localization of tubulin and microtubule-associated protein 2 during the development of hippocampal neurons in culture.  J. Neurosci. 6:714‑722.

Dotti, C.G, Banker, G.A, and Binder, L.I. (1987) The expression and distribution of the microtubule-associated proteins tau and microtubule-associated protein 2 in hippocampal neurons in situ and in cell culture.  Neuroscience 23: 121‑130. 

Dotti, C.G., and Banker, G.A. (1987) Experimentally induced alteration in the polarity of developing neurons.  Nature 330:254‑256.

Davis, L., Banker, G., and Steward, O. (1987) Selective dendritic transport of RNA in hippocampal neurons in culture.  Nature 330:477‑479.

Higgins, D., Waxman, A., and Banker, G., (1988) The distribution of microtubule-associated protein 2 changes when dendritic growth is induced in rat sympathetic neurons in vitro.  Neuroscience 24:583‑592.

Dotti, C.G., Sullivan, C.A., and Banker, G.A. (1988) The establishment of polarity by hippocampal neurons in culture.  J. Neurosci. 8:1454‑1468.

Baas, P.W., Deitch, J.S., Black, M.M., and Banker, G.A. (1988)  Polarity orientation of microtubules in hippocampal neurons:  uniformity in the axon and nonuniformity in the dendrite.  Proc. Natl. Acad. Sci. U.S.A. 85:8335‑8339.

Goslin, K., Schreyer, D.J., Skene, J.H.P., and Banker, G. (1988) Development of neuronal polarity:  GAP‑43 distinguishes axonal from dendritic growth cones.  Nature 336:672‑674

Goslin, K. and Banker, G. (1989) Experimental observations on the development of polarity by hippocampal neurons in culture.  J. Cell Biol. 108:1507‑1516.

Goslin, K., Birgbauer, E., Banker, G., and Solomon, F. (1989) The role of cytoskeleton in organizing growth cones: a microfilament-associated growth cone component depends upon microtubules for its localization.  J. Cell Biol. 109:1621‑1631.

Baas, P.W., Black, M.M., and Banker, G.A. (1989)  Changes in microtubule polarity orientation during the development of hippocampal neurons in culture.  J. Cell Biol. 109:3085‑3094.

Fletcher, T., and Banker, G. (1989)  The establishment of polarity by hippocampal neurons: the relationship between the stage of a cell's development in situ and its subsequent development in culture.  Devel. Biol. 136:446‑455.

Goslin, K., Schreyer, D.J., Skene, J.H.P., and Banker, G. (1990)  Changes in the distribution of GAP‑43 during the development of neuronal polarity.  J. Neurosci. 10:588‑602.

Goslin, K., and Banker, G. (1990)  Rapid changes in the distribution of GAP‑43 correlate with the expression of neuronal polarity during normal development and under experimental conditions.  J. Cell Biol. 110: 1319‑1332.

Davis, L., Burger, B., Banker, G., and Steward, O. (1990) Dendritic transport:  quantitative analysis of the time course of somatodendritic transport of recently synthesized RNA.  J. Neurosci. 10: 3056‑3068.

Kleiman, R., Banker, G., and Steward, O. (1990)  Differential subcellular localization of particular mRNAs in hippocampal neurons in culture.  Neuron 5: 821‑830.

Fletcher, T., Cameron, P., De Camilli, P., and Banker, G.  (1991)  The distribution of synapsin I and synaptophysin in hippocampal neurons developing in culture.  J. Neurosci. 11: 1617‑1626.

Dotti, C.G., and Banker, G. (1991)  Intracellular organization of hippocampal neurons during the development of neuronal polarity.  J. Cell Sci. Suppl. 15: 75‑84.

Kleiman, R., Banker, G., and Steward, O. (1992) Temperature-dependent blockade of nucleocytoplasmic transport of newly synthesized RNA in neurons.  Molec. Brain Res., 13: 103‑109.

Ferreira, A., Niclas, J., Vale, R., Banker, G., and Kosik, K. (1992)  Suppression of kinesin expression in cultured hippocampal neurons using antisense oligonucleotides.  J. Cell Biol. 117: 595‑606.

Lein, P., Banker, G., and Higgins, D. (1992) Laminin selectively enhances axonal growth and accelerates the development of polarity by hippocampal neurons in culture.  Devel. Brain Res. 69: 191-197.

Pietrini, G., Matteoli, M., Banker, G., and Caplan, M.J. (1992) Isoforms of the Na,K-ATPase are present in both axons and dendrites of hippocampal neurons in culture.  Proc. Natl. Acad. Sci. U.S.A. 89: 8414-8418.

Craig, A.M., Blackstone, C.D., Huganir, R.L., and Banker, G. (1993) Distribution of non-NMDA glutamate receptor subunits in cultured rat hippocampal neurons: development of a postsynaptic receptor mosaic.  Neuron 10: 1055-1068.

Deitch, J.S., and Banker, G. (1993) An electron microscopic analysis of hippocampal neurons developing in culture:  early stages in the development of polarity.  J. Neurosci. 13: 4301-4315.

Kleiman, R., Banker, G., and Steward, O. (1993) Subcellular distribution of rRNA and polyA RNA in hippocampal neurons in culture.  Molec. Brain Res. 20: 305-312.

Kleiman, R., Banker, G., and Steward, O. (1993) Inhibition of protein synthesis alters the subcellular distribution of mRNA in neurons, but does not prevent dendritic transport of RNA.  Proc. Natl. Acad. Sci. U.S.A. 90: 11192-11196.

Kleiman, R., Banker, G., and Steward, O. (1993) Development of subcellular mRNA compartmentation in hippocampal neurons in culture.  J. Neurosci. 14: 1130-1140.

Benson, D.L., Watkins, F.H., Steward, O., and Banker, G. (1994)  Characterization of GABAergic neurons in hippocampal cell cultures.  J. Neurocytol. 23: 279-295.

Fletcher, T.L., De Camilli, P., and Banker, G.  (1994)  Synaptogenesis in hippocampal cultures: Axons and dendrites become competent to form synapses at different stages of development.  J. Neurosci. 14: 6695-6706.

Craig, A.M., Blackstone, C.D., Huganir, R.L., and Banker, G. (1994) Selective clustering of glutamate and GABAA receptors opposite terminals releasing the corresponding neurotransmitters. Proc. Natl. Acad. Sci. U.S.A. 91: 12373-12377.

Mandell, J.W., and Banker, G.  (1995)  The microtubule cytoskeleton and the development of neuronal polarity.  Neurobiol. Aging 16: 229-238.

Craig, A.M., Wyborski, R.J., and Banker, G. (1995)  Preferential addition of newly synthesized membrane proteins at axonal growth cones.  Nature (Lond.) 375: 592-594.

Benson, D.L., Mandell, J.W., Shaw, G., and Banker, G. (1996)  Compartmentation of alpha-internexin and neurofilament triplet proteins in cultured hippocampal neurons. J. Neurocytol.25: 181-196.

Craig, A.M., Banker, G., Chang, W., McGrath, M.E., and Serpinskaya, A.S. (1996)  Clustering of gephyrin at GABAergic but not glutamatergic synapses in cultured rat hippocampal neurons. J. Neurosci. 16: 3166-3177.

Mandell, J. W. and Banker, G. (1996)  A spatial gradient of tau protein phosphorylation in nascent axons.  J. Neurosci. 16: 5727-5740.

Brown, M., Banker, G.A., Hussaini, I.M., Gonias, S.L., and VandenBerg, S.R. (1996)  Low density lipoprotein receptor-related protein is expressed early and becomes restricted to the somatodendritic domain during neuronal differentiation in culture.  Brain Res. 747: 313-317

Withers, G.S., George, J.M., Banker, G.A., and Clayton, D.F. (1997) Delayed localization of synelfiin (synuclein, NACP) to presynaptic terminals in cultured hippocampal neurons.  Develop. Brain Res. 99: 87-94.

Jareb, M. and Banker, G. (1997) Inhibition of axonal outgrowth by brefeldin A in cultured hippocampal neurons.  J. Neurosci. 7: 8955-8963.

Mandell, J. and Banker, G. (1998)  Selective blockade of axonogenesis in cultured hippocampal neurons by the tyrosine phosphatase inhibitor, orthovanadate.  J. Neurobiol. 35:17-28.

Ruthel, G. and Banker, G. (1998) Actin-dependent anterograde movement of growth‑cone‑like structures along growing hippocampal axons: a novel form of axonal transport?  Cytoskeleton and Cell Motility 40:160-173.

Jareb, M. and Banker, G. (1998) The polarized sorting of membrane proteins expressed in cultured hippocampal neurons using viral vectors.  Neuron 20: 855-867.

Muslimov, I.A., Banker, G., Brosius, J., and Tiedge, H.  (1998) Activity-dependent regulation of dendritic BC1RNA in hippocampal neurons in culture.  J. Cell Biol 141: 1601-1611.

Craighead, H.G., Turner, S.W., Davis, R.C., James, C., Perez, A.M., St. John, P.M., Isaacson, M.S., Kam, L., Shain, W., Turner, J.N., and Banker, G. (1998) Chemical and topographic surface modification for control of central nervous system cell adhesion.  J. Biomed. Devices 1: 49-64.

Ruthel, G. and Banker, G. (1999)  The role of moving growth-cone-like “wave” structures in the outgrowth of cultured hippocampal axons and dendrites. J Neurobiol. 39:97‑106.

Esch, T., Lemmon, V., and Banker, G.  (1999) Local presentation of substrate molecules directs axon specification by cultured hippocampal neurons.  J Neurosci. 19:6417‑26.

Shogomori,  H., Burack,  M.A., Banker, G., Futerman, A.H.  (1999)  Gangliosides GM1 and GD1b are not polarized in mature hippocampal neurons.  FEBS Lett 458:107‑111.

James, C.D., Davis, R.C., Meyer, M., Perez, A., Turner, S., Withers, G., Kam, L. Banker, G., Craighead, H.G., Isaacson, M., Turner, J.N., Shain, W. (2000) Aligned microcontact printing of micrometer scale polylysine structures for controlled growth of cultured neurons on planar microelectrode arrays.  IEEE Transactions in Biomedical Engineering 47: 17-21.

Withers, G.S., Higgins, D., Charette, M., and Banker, G. (2000) Bone morphogenetic protein-7 increases dendritic growth and receptivity to innervation in cultured hippocampal neurons.  Europ. J. Neurosci. 12:106‑116.

Grewal, S.S., Horgan, A.M., York, R.D., Withers, G.S., Banker, G.A., Stork, P.J. (2000) Neuronal calcium activates a rap1 and B‑Raf signaling pathway via the cyclic adenosine monophosphate‑dependent protein kinase.   J. Biol. Chem. 275:3722‑3728

Burack, M.A., Silverman, M.S., and Banker, G. (2000)  The role of selective transport in neuronal protein sorting.  Neuron 26: 465-472.  

Esch, T., Lemmon, V., and Banker, G. (2000)  Differential effects of NgCAM and N-cadherin on the development of axons and dendrites by cultured hippocampal neurons.  J. Neurocytol. 29: 215-223.

Silverman, M.A.,  Kaech, S.,  Jareb, M.,  Burack, M.A., Vogt, L., Sonderegger, P., and Banker, G.  (2001)  Sorting and directed transport of membrane proteins during the development of hippocampal neurons in culture.  Proc. Natl. Acad. Sci. U.S.A. 98:7051‑7057.

Cheng, C., Glover, G., Banker, G., and Amara, S.  (2002)  A novel sorting motif in the glutamate transporter Excitatory Amino Acid Transporter 3 directs its targeting in Madin-Darby canine kidney cells and hippocampal neurons.  J. Neurosci. 22: 10643-52.

Sampo, B., Kaech, S., Kunz, S., and Banker, G. (2003)  Two distinct mechanisms target membrane proteins to the axonal surface.  Neuron 37:611-24.

Oliva, A.A.Jr., James, C.D., Kingman. C.E., Craighead, H.C., and Banker, G.A. (2003)  Patterning axonal guidance molecules using a novel strategy for microcontact printing.  Neurochem. Res. 28:1639-1648.

Wayman, G., Kaech, S., Grant, W., Davare, M., Impey, S., Tokumitsu, H., Nozaki, N., Banker, G., and Soderling, T.  (2004)  Regulation of axonal extension and growth cone motility by Calmodulin-dependent Protein Kinase I.  J. Neurosci. 24: 3786-94.

附录:GARY实验室一些非常精彩的动画(有关AXON生长,蛋白转运等).

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