Curriculum vitae J. Klumperman

Family name:   Klumperman

First name:   Judith

Sex:    female

Work address:   Cell Microscopy Centre
    Department of Cell Biology,
    University Medical Center Utrecht (UMCU),
    AZU G02.525, Heidelberglaan 100
    3584CX Utrecht, The Netherlands
    Phone: 31-30-2506550/6551
    Fax: 31-30-2541797
    Email: J.Klumperman@lab.azu.nl

Date of birth:   August 9, 1961

Place of birth:   Delft, The Netherlands

Nationality:   Dutch

Start university training:  September 1979, University of Leiden

Discipline:   Biology

Graduate:   April 1985

PhD:    May 1990

Employment record
April 1985-September 1989: PhD at the Laboratory for Electron Microscopy, Medical Faculty, University of Leiden (UL), The Netherlands (Promotor: Prof. W. T. Daems) and the Department for Biochemistry, Academic Medical Centre, University of Amsterdam, The Netherlands (Promotor: Prof. J.M. Tager).
PhD thesis: Transport of lysosomal and brush border enzymes in polarized colon carcinoma cell lines.
September 1989-October 1992: Post-doctoral fellowship at the Department of Cell Biology, Center for Electron Microscopy, Medical Faculty, University of Utrecht, the Netherlands. (Supervisor: Prof. H.J. Geuze) and Prof. K. Von Figura, Department of Biochemistry, Georg-August University, Göttingen, Germany.
October 1992-July 1997: Assistant professor (tenure) at the Department of Molecular and Cellular Neurobiology, Faculty Biology, Free University (VU), Amsterdam, The Netherlands.
July 1997: Assistant professor (tenure) at the Department of Cell Biology, University Medical Centre Utrecht (UMCU), The Netherlands.
July 1999-July 2001: Associate professor (tenure) at the Department of Cell Biology, UMCU, The Netherlands.
July 2001-present: Full professor Cell Biology and Head of the Cell Microscopy Centre, Department of Cell Biology, UMCU, The Netherlands.

Professional Societies
Dutch Society for Microscopy
Dutch Society for Cell Biology
American Society for Cell Biology

 

Research interest
My general research interest is to understand the mechanisms of vesicular trafficking in relation to organelle formation and functioning in healthy and diseased cells. My current research focuses on lysosome biogenesis. Lysosomes have long been considered as the terminal degradative compartments of the cell, but this view is now completely changed by an overwhelming amount of data demonstrating multiple specialized functions of lysosomes. Resolving the pathways of lysosome assembly is therefore eminent to understand the molecular basis of the growing group of genetic disorders that are converted to lysosomal dysfunctions.
My research has added to the functional characterization of transport-machinery proteins, e.g. Rab , coat- and SNARE proteins, in physiological and pathological conditions. Two important milestones in my work are 1. the ‘concentration-by-exclusion’ model for transport of secretory proteins from the ER to the Golgi complex and 2. establishing the presence of Golgi-enzymes in Golgi-associated COPI vesicles. Both findings have important implications for understanding the mode of transport through this complex part of the secretory pathway. In addition, my work contributed to the identification of the mannose 6-phosphate receptor-pathway for lysosomal enzyme delivery and the discovery of a novel, AP-3 mediated pathway that transports lysosomal membrane proteins from tubular sorting endosomes to lysosomes.
Highlights in my work on the endocytic pathway are the identification of a Hrs/clathrin-coat on endosomes (the precursors of lysosomes) that is necessary for the concentration and sorting of growth factor receptors to the lysosomal degradation pathway. We are currently extending these studies to specific types of breast cancer, in which receptor degradation is impaired resulting in continuous signaling for growth.
During the course of my career I have specialized in the application of microscopy methods, especially immuno-electron microscopy, to address biomedical questions in the membrane traffic field. From 2001 on I am Director of the internationally renowned Cell Microscopy Center for the application of advanced microscopy studies in cutting edge bio-medical research. During my studies I have experienced the limitations of a static approach to study dynamic events. Recently, I introduced a correlative microscopy method that allows a direct combination of live-cell imaging with the most sensitive immunoEM method to date.

Management
1989-1990: Board member of the Department of Cell Biology, Medical Faculty, University of Utrecht.
1995-1997: Board member of the Department of Molecular and Cellular Neurobiology, Faculty Biology, Free University Amsterdam.
1998-present: Member of the daily board/management team of the Department of Cell Biology, UMCU.
2000: Interim-project leader at the Graduate School Institute of Biomembranes: Membrane specificity and organelle biogenesis.
2001: Project leader at the Graduate School Institute of Biomembranes: Membrane specificity and organelle biogenesis.
2001: Chair of the Cell Microscopy Centre, UMC Utrecht
2001: Board member of the Dutch Society for Microscopy
2002-2006: Member of the Council for Medical Sciences (Raad voor Medische Wetenschappen) of the Royal Dutch Academy of Arts and Sciences (KNAW).
2002-2004: member of the committee: Financing biomedical research. Royal Dutch Academy of Arts and Sciences (KNAW).
June 2002-present: Vice-president of the Dutch Society for Microscopy
2004: Member of the committee ‘Internationalisering’. Strategic impulse, UMC Utrecht.
2004: Board member Graduate School Institute of Biomembranes:
2004: Member of the Talma Eykman prize jury.
2004: Chair of the board of the Graduate School Institute of Biomembranes

Organization International Conferences
1999: Co-organiser of the international mini-symposium: ‘Cell Biology of Disease’. AZU-UMCU, Utrecht, The Netherlands.
2000: Co-organizer and chair of the mini-symposium ‘Membrane Traffic’ of the European Electron Microscopy congress EUREM 2000, Brno, Czech Republic, July 9-14, 2000.
2001: Co-organizer of and chair at the international symposium Molecular mechanisms in membrane transport. June 28-29, Utrecht, The Netherlands.
2001: Vice-president of the organizing committee of the annual meeting of the Dutch Society for Microscopy (NVvM). December 13-14, Papendal Conference Center, The Netherlands
2002: Member of the organizing committee of the 44th International Symposium of the Society for Histochemistry ‘Proteomics in situ: imaging proteins at work’. Vlissingen, 25-28 September 2002, The Netherlands.
2002: Co-organizer and chair of the mini-symposium ‘Organelle Biogenesis and Inheritance’ at the 42nd Annual Meeting of the American Society for Cell Biology (ASCB). 14-18 December 2002, San Francisco, USA.
2004: Chair mini-symposium: Transport through the Golgi apparatus. Keystone symposium: Golgi apparatus and secretory pathway of eukaryotic cells. January 20 – january 25, Beaver Run Resort, Breckenridge, Colorado. Organizers: L.A. Staehelin, B. Glick & K.E. Howell
2004: Member of the organizing committee of the Royal Dutch Academy of Arts and Sciences (KNAW) symposium: Financing biomedical research in the Netherlands (De financiering van (bio) medisch wetenschappelijk onderzoek in Nederland). June 17. Amsterdam, The Netherlands.
2004: Member of the Scientific Advisory Board of the 2004 Electron Microscopical Society (EMC) Congress. August 22-27. Antwerpen, Belgium
2004: Member of the Programme committee of the 2004 European Life Science Organization (ELSO) annual meeting. September 4-8. Nice, France.

Other academic activities
- Reviewer for: Journal of Cell Biology, EMBO Journal, Nature Cell Biology, Molecular Biology of the Cell, Journal of Cell Science, Traffic, Histochemistry & Cell Biology.
- Grant reviewer for: the Dutch Society for Scientific research (NWO), Wellcome Trust (UK), Medical Research Council (UK), Telethon (Italy), KNAW.
1996-1997: Co-organiser of the weekly seminar series at the Department of Molecular and Cellular Neurobiology, Faculty Biology, Free University, Amsterdam.
1997: Member of the organising committee of the AIO/OIO-meeting of the Research Institute Neurosciences Amsterdam.
June 1999: J. Klumperman. Arts and artifacts of electron microscopy. Trends in Cell Biology, 1999, Vol. 9:247. Review on ‘Biomedical Electron Microscopy, Illustrated methods and Interpretations, by A.B. Maunsbach and B.A. Afzelius, ISBN0124806104. (on invitation)
February 2000: Cover design of Molecular Biology of the Cell, the international journal of the American Society for Cell Biology (ASCB), Vol. 11, No. 2. (on invitation)
July 2000: J. Klumperman. Immuno-electronenmicroscopie, Analyse, vakblad Nederlandse Vereniging van bio-Medische Laboratoriummedewerkers. November 2000, 270-274. (on invitation).
2001: Corresponding jury member of the committee ‘Stichting Electronenmikroskopie Nederland (SEN)’ (= Dutch society for Electron microscopy) awarding the SEN-prize 2001.

Editorships
2002-present: Traffic
2002-present: Histochemistry and Cell Biology
2003-present: Biology of the Cell
2004: Section editor of the Membranes and organelles (August 2004) issue of Current Opinion in Cell Biology

Grants/prizes
1996: Grant 805-26.183 of the Netherlands Organization of Scientific Research (NWO/Stichting Levenswetenschappen): Molecular mechanisms of neuronal membrane traffic in synaptic vesicle recycling.
1998: UMCU-grant: Attraction and maintenance of female talent.
2000: Grant 8PJ/00-31 of the Netherlands Organization of Scientific Research (NWO/ALW): Identification and characterization of vesicular transport intermediates in axons.
2000: FOM Grant FBI-IIEOI/00-11: Electron tomography and 3D-analysis of intracellular compartments: The early biosynthetic pathway. With Dr. B. Koster (UU)
2001: Genentech, Inc., San Francisco, USA. Purchase order No. 559963. Project: Intracellular trafficking of Tumor Antigens and their Binding Antibodies.
2002: ISAO Award (International Foundation Alzheimer Research): Subcellular localization of the active g-secretase complex. Border crossing research grant with Prof. W. Annaert, University of Leuven.
2003: ZonMW investment grant: Advanced Live-cell Imaging station for multipurpose biomedical imaging.
2004: Intra-European Marie-Curie Fellowship (Motility 5): Defining the transport pathway of the receptor-mediated sorting of lysosomal enzymes by correlative live-cell imaging and electron-microscopy.
2004: ZonMW VICI award: Defining novel pathways of lysosome biogenesis: a genome-wide screen combined with correlative microscopy.
2005: Horizon Breakthrough grant. A semi-automated fluorescence microscopy-based RNA-interference screen to define novel pathways of lysosome biogenesis.
2005: internationaliserings subsidie

List of publications

1. Tager, J.M., R.P. Oude Elferink, A. Reuser, M. Kroos, L.A. Ginsel, J.A. Fransen, and J. Klumperman. 1987. Alpha-glucosidase deficiency (Pompe’s disease). [Review]. Enzyme 38:280-285.

2. Fransen, J.A., L.A. Ginsel, P.H. Cambier, J. Klumperman, R.P. Oude Elferink, and J.M. Tager. 1988. Immunocytochemical demonstration of the lysosomal enzyme alpha-glucosidase in the brush border of human intestinal epithelial cells. Eur. J. Cell Biol. 47:72-80.

3. Eilers, U., J. Klumperman, and H.P. Hauri. 1989. Nocodazole, a microtubule-active drug, interferes with apical protein delivery in cultured intestinal epithelial cells (Caco-2). J. Cell Biol. 108:13-22.

4. Oude Elferink, R.P., J.A. Fransen, J. Klumperman, L.A. Ginsel, and J.M.. Tager. 1989. Secretion of a precursor form of lysosomal alpha-glucosidase from the brush border of human kidney proximal tubule cells. Eur. J. Cell Biol. 50:299-303.

5. Matter, K., B. Stieger, J. Klumperman, L.A. Ginsel, and H.P. Hauri. 1990. Endocytosis, recycling, and lysosomal delivery of brush border hydrolases in cultured human intestinal epithelial cells (Caco-2). J. Biol. Chem. 265:3503-3512.

6. Klumperman, J., J.C. Boekestijn, A.M. Mulder, J.A. Fransen, and L.A. Ginsel. 1991. Intracellular localization and endocytosis of brush border enzymes in the enterocyte-like cell line Caco-2. Eur. J. Cell Biol. 54:76-84.

7. Klumperman, J., J.A. Fransen, J.C. Boekestijn, R.P. Oude Elferink, K. Matter, H.P. Hauri, J.M. Tager, and L.A. Ginsel. 1991. Biosynthesis and transport of lysosomal alpha-glucosidase in the human colon carcinoma cell-line Caco-2: secretion from the apical surface. J. Cell Science 100:339-347.

8. Damke, H., J. Klumperman, K. von Figura, and T. Braulke. 1991. Effects of brefeldin A on the endocytic route. Redistribution of mannose 6-phosphate/insulin-like growth factor II receptors to the cell surface. J. Biol. Chem. 266:24829-24833.

9. Damke, H., J. Klumperman, K. von Figura, and T. Braulke. 1992. Brefeldin A affects the cellular distribution of endocytic receptors differentially. Biochem. Biophys. Res. Comm. 185:719-727.

10. Hille, A., J. Klumperman, H.J. Geuze, C. Peters, F.M. Brodsky, and K. von Figura. 1992. Lysosomal acid phosphatase is internalized via clathrin-coated pits. Eur. J. Cell Biol. 59:106-115.

11. Klumperman, J., J.A. Fransen, J.M. Tager, and L.A. Ginsel. 1992. The cation-independent mannose 6-phosphate receptor is not involved in the polarized secretion of lysosomal alpha-glucosidase from Caco-2 cells. Eur. J. Cell Biol. 57:147-154.

12. Klumperman, J., A. Hille, T. Veenendaal, V. Oorschot, W. Stoorvogel, K. von Figura, and H.J. Geuze. 1993. Differences in the endosomal distributions of the two mannose 6-phosphate receptors. J. Cell Biol. 121:997-1010.

13. Klumperman, J., J. Krijnse-Locker, A. Meijer, M.C. Horzinek H.J. Geuze and P.J. Rottier, 1994. Coronavirus M proteins accumulate in the Golgi complex beyond the site of virion budding. J. Virology 68:6523-6534.

14. Krijnse-Locker, J., J. Klumperman, V. Oorschot, M.C. Horzinek, H.J. Geuze and P.J. Rottier. 1994. The cytoplasmic tail of mouse hepatitis virus M protein is essential but not sufficient for its retention in the Golgi complex. J. Biol. Chem. 269:28263-28269.

15. Itin, C., M. Foguet, F. Kappeler, J. Klumperman, and H.P. Hauri. 1995. Recycling of the endoplasmic reticulum/Golgi intermediate compartment protein ERGIC53 in the secretory pathway. Biochem. Soc. Transactions 23:541-544.

16. Klumperman, J., S. Spijker, J. van Minnen, H. Sharp-Baker, A.B. Smit, and W.P.M. Geraerts. 1996. Cell type specific sorting of neuropeptides: a mechanism to modulate peptide composition of large dense core vesicles. J. of Neuroscience 15:7930-7940.

17. Kuliawat, R., J. Klumperman, L. Ludwig, and P. Arvan. 1997. Differential sorting of lysosomal enzymes out of the regulated secretory pathway in pancreatic beta cells. J. Cell Biol. 137:595-608.

18. Bock, J., J. Klumperman, S. Davanger, and R.H. Scheller. 1997. Syntaxin 6 functions in trans-Golgi network vesicle trafficking. Molc. Biol of the Cell.8:1261-1271.

19. Feng, Z., Klumperman, J., Lukowiak, K. and N.I. Syed. 1997. In vitro synaptogenesis between the somata of identified Lymnaea neurons requires proteins synthesis but not extrinsic growth factors or substrate adhesion molecules. J. Neuroscience 17:7839-7849.

20. Dell’Angelica, E.C., J. Klumperman, W. Stoorvogel, and J.S. Bonaficino. 1998. Association of the AP-3 adaptor complex with clathrin. Science 280:431-434.

21. Klumperman, J., R. Kuliawat, J. Griffith, H.J. Geuze and P. Arvan. 1998. Mannose 6-phosphate receptors are sorted from immature secretory granules via AP-1, clathrin and syntaxin 6-positive vesicles. J. Cell Biol.141:359-371.

22. Hay*, J.C., Klumperman*, J., V. Oorschot, M. Steegmaier, C.S. Kuo and R.H. Scheller. 1998. Localization, dynamics, and protein interactions reveal distinct roles for ER and Golgi SNAREs. J. Cell Biol. 141:1489-1502. *with equal contributions.

23. Klumperman, J., A. Schweizer, H. Clausen, B.L. Tang, W. Hong, V. Oorschot, and H.P. Hauri. 1998. The recycling pathway of protein ERGIC-53 and dynamics of the ER-Golgi intermediate compartment. J Cell Science 111:3411-3425

24. Prekeris, R., J. Klumperman, Y.A. Chen, and R.H. Scheller. 1998. Syntaxin 13 mediates cycling of plasma membrane proteins via tubulovesicular recycling endosomes. J. Cell Biol. 143:957-971.

25. Spencer, GE, J. Klumperman, and NI Syed. 1998. Neurotransmitters and neurodevelopment. Role of dopamine in neurite outgrowth, target selection and specific synapse formation. Perspect Dev Neurobiol. 5:451-467. Review.

26. Chao, D., J.C. Hay, S. Winnick, R. Prekeris, J. Klumperman and R.H. Scheller. 1999. SNARE Membrane trafficking dynamics in vivo. J. Cell Biol. 144:869-881.

27. Steegmaier, M., J. Klumperman, D.L. Foletti, J. Yoo and R.H. Scheller. VAMP4 functions in trans-Golgi network vesicle trafficking. Molc. Biol. of the Cell 10:1957-1972, 1999.

28. Martinez-Menarguez, J., H.J. Geuze, J.W. Slot and J. Klumperman. Vesicular tubular clusters between ER and Golgi mediate concentration of soluble secretory proteins by exclusion from COPI-coated vesicles. Cell 98:81-90, 1999.

29. Advani, R.J., B. Yang, R. Prekeris, K.C. Lee, J. Klumperman, and R.H. Scheller. 1999. VAMP7 mediates vesicular transport from endosomes to lysosomes. J. Cell Biol.146:765-775.

30. de Wit, H., Y. Lichtenstein, H.J. Geuze, R.B. Kelly, P. van der Sluijs and J. Klumperman. 1999. Synaptic vesicles form by budding from tubular extensions of sorting endosomes in PC12 cells. Molc. Biol. of the Cell 10: 4163-4176.

31. Prekeris, R., B. Yang, V. Oorschot, J. Klumperman, and R.H. Scheller. 1999. Differential roles of syntaxin 7 and syntaxin 8 in endosomal trafficking. Molc. Biol. of the Cell 10:3891-3908.

32. Dittie, A., J. Klumperman and S. Tooze. 1999. Mannose-6-phosphate receptors are present on a subset of immature secretory granules in PC12 cells. J. Cell Science 112:3955-3966.

33. Huang, C.S. J. Zhou, A.K. Feng, C.C. Lynch, J. Klumperman, S.J. DeArmond and W.C. Mobley. 1999. NGF signaling in caveolae-like domains at the plasma membrane. J. Biol. Chem. 274: 36707-36714.

34. Krantz, D.E., Y. Liu, R.I. Wilson, P.K. Tan, V. Oorschot, J. Klumperman, and R.H. Edwards. 2000. Phosphorylation regulates sorting of the vesicular acetylcholine transporter to different classes of secretory vesicles. J. Cell Biol. 149:379-395.

35. Steegmaier, M., V. Oorschot, J. Klumperman, and R.H. Scheller. 2000. Syntaxin 17 is abundant in steroidogenic cells and implicated in smooth endoplasmic reticulum membrane dynamics. Molc. Biol. of the Cell. 11:2719-2731.

36. Klumperman, J. 2000. Transport between ER and Golgi. [Review]. Current Opinion in Cell Biology 12:445-449.

37. Prekeris, R., J. Klumperman, and R.H. Scheller. 2000. Syntaxin 11 is an atypical SNARE abundant in the immune system. Eur. J. Cell Biology 79, 771-780.

38. J. Klumperman. The growing Golgi: in search of its independence. 2000. Nature Cell Biol. 2, E217-E218.

39. Prekeris, R., J. Klumperman, and R.H. Scheller. 2000. A Rab11/Rip11 protein complex regulates apical membrane trafficking via recycling endosomes. Molecular Cell 6, 1437-1448.

40. Pende, M., S.C. Kozma, M. Jacquet, V. Oorschot, R. Burcelin, Y. Le Marchand-Brustel, J. Klumperman, B. Thorens, and G. Thomas. 2000. Hypoinsulinaemia, glucose intolerance and diminished b-cell size in S6K1-deficient mice. Nature 408, 994-997.

41. Van Kerkhof, P., M. Sachse, J. Klumperman, and G. Strous. 2001. Growth hormone receptor ubiquitination coincides with recruitment to clathrin-coated membrane domains. J. Biol. Chem. 27, 3778-3784.

42. Smit AB, NI Syed, D Schaap, J. van Minnen, J. Klumperman, KS Kits, H. Lodder, RC van der Schors, R. van Elk, B. Sorgedrager, K. Brejc, TK Sixma, WP Geraerts. 2001. A glia-derived acetylcholine-binding protein that modulates synaptic transmission. Nature 411, 261-268. (impact factor

43. de Wit, H., Y. Lichtenstein, R.B. Kelly, H.J. Geuze, J. Klumperman*, and P. van der Sluijs*. 2001. Rab4 regulates formation of synaptic-like microvesicles and constitutive recycling vesicles from early endosomes in PC12 cells. Mol Biol Cell. 12 (11):3703-3715. *with equal contributions

44. Sachse, M., P. van Kerkhof, G. Strous, and J. Klumperman. 2001. The ubiquitin-dependent endocytosis motif is required for the incorporation of growth hormone receptor in the forming clathrin-coated pit. J. Cell Science 114 (21):3943-3952.

45. Oprins, A, C. Rabouille, G. Posthuma, J. Klumperman, H.J. Geuze and J.W. Slot. 2001. The ER to Golgi interface is the major site of secretory protein concentration in exocrine pancreatic cells. Traffic 2:831-838.

46. Sprong, H., S. Degroote, T. Claessens, J. van Drunen, V. Oorschot, B.H.. Westerink, Y. Hirabayashi, J. Klumperman, P. van der Sluijs, and G. van Meer. 2001 Glycosphingolipids are required for sorting melanosomal proteins in the Golgi complex. J Cell Biol. 155 (3):369-380.

47. Klopfenstein DR, J. Klumperman, A. Lustig, RA Kammerer, V. Oorschot, and HP Hauri.  2001. Subdomain-specific localization of CLIMP-63 (p63) in the endoplasmic reticulum is mediated by its luminal alpha-helical segment. J Cell Biol. 153 (6):1287-1300.

48. van Kerkhof P, CM dos Santos, M. Sachse, J. Klumperman, G. Bu, and GJ Strous. 2001. Proteasome Inhibitors Block a Late Step in Lysosomal Transport of Selected Membrane but not Soluble Proteins. Mol Biol Cell. 12 (8):2556-2566.

49. Martinez-Menarguez, J.A., R. Prekeris, V. Oorschot, RH Scheller, JW Slot, HJ Geuze, and J. Klumperman. 2001. Peri-Golgi vesicles contain retrograde but not anterograde proteins consistent with the cisternal progression model of intra-Golgi transport. J Cell Biol. 155: 1213-1224.

50. Sachse, M., G. Ramm, G. Strous, and J. Klumperman. 2002. Endosomes: multipurpose designs for integrating housekeeping and specialized tasks. [Review]. Histochem. and Cell Biol. 117:91-104

51. Sachse M., S. Urbé, V. Oorschot, G. Strous, and J. Klumperman. 2002. Bi-layered clathrin coats on endosomal vacuoles are involved in protein sorting towards lysosomes. Molc. Biol. Cell. 13:1313-1328.

52. Mohrmann K., L. Gerez, V. Oorschot, J. Klumperman and P. van der Sluijs. 2002. Rab4 function in membrane recycling from early endosomes depends on a membrane to cytoplasm cycle. J. Biol. Chem. 277:32029-3325.

53. Oorschot V., de Wit, H., Annaert, W and Klumperman, J. 2002. A novel flat-embedding method to prepare ultrathin cryosections from cultured cells in their in situ orientation. J. Histochem. Cytochem 50:1067-1080.

54. Andrieux A, Salin PA, Vernet M, Kujala P, Baratier J, Gory-Faure S, Bosc C, Pointu H, Proietto D, Schweitzer A, Denarier E, Klumperman J, Job D. 2002. The suppression of brain cold-stable microtubules in mice induces synaptic defects associated with neuroleptic-sensitive behavioral disorders. Genes Dev. 16:2350-2364.

55. Deneka M, Neeft M, Popa I, van Oort M, Sprong H, Oorschot V, Klumperman J, Schu P, van der Sluijs P. 2003. Rabaptin-5alpha/rabaptin-4 serves as a linker between rab4 and gamma(1)-adaptin in membrane recycling from endosomes. EMBO J. 22(11):2645-2657.

56. Rechards, M., Xia, W., Oorschot, VM, Selkoe DJ and J. Klumperman. 2003. Presenilin-1 Exists in Both Pre- and Post-Golgi Compartments and Recycles Via COPI-Coated Membranes. Traffic 4(8):553-565.

57. Urbé, S., Sachse, M., Row, P.E., Preisinger, C., Barr, F.A., Strous, G., Klumperman, J., and Clague, M.J. 2003. The UIM domain of Hrs couples receptor sorting to vesicle formation. J Cell Sci. 116:4169-4179.

58. A.J. Koster and J. Klumperman. Electron microscopy: stretching the resolution limits of imaging (Review). 2003. Nature Review Mol Cell Biology/Nature Cell Biology. Supplement September issue:ss6-ss10 (IF = 21/15)

59. Hendriks G, Koudijs M, Van Balkom BW, Oorschot V, Klumperman J, Deen PM, Van Der Sluijs P. 2004. Glycosylation is important for cell surface expression of the water channel aquaporin-2, but is not essential for tetramerization in the endoplasmic reticulum. J Biol Chem. 279:2975-2983. Epub 2003 Oct 30.

60. Sachse, M., Strous, G.J. and J. Klumperman. 2004. ATPase deficient hVPS4 impairs formation of internal endosomal vesicles and stabilizes bilayered clathrin coats on endosomal vacuoles. J. Cell Science. 117:1699-1708. Epub 2004 Mar 09.

61. Bouwman, J., Maia, A.S., Camoletto, P., Posthuma, G., Roubos, E.W., Oorschot, V.M.J., Klumperman, J. and M. Verhage. 2004. Quantification of synapse formation and maintenance in vivo in the absence of synaptic release. Neuroscience 126:115-126.

62. Junutala, J.R., De Mazière, A., Peden, A.A., Ervin, K.E., Advani, R.J., van Dijk, S.M., Klumperman, J. and R.H. Scheller. 2004.  Rab14 is involved in membrane trafficking between the Golgi complex and endosomes. Molc. Biol. Cell. 15:2218-2229.

63. Griffith, G. and J. Klumperman. 2004. Membranes and sorting. Current Opinion in Cell Biology 16:341-342. (review on invitation).

64. Peden, A.A., Oorschot, V., Hesser, B., Austin, C.D., Scheller, R.H. and J. Klumperman. 2004. Localization of the AP-3 adaptor complex defines a novel endosomal exit site for lysosomal membrane proteins. J. Cell Biol. 164:1065-1076.

65. Esselens, C., Oorschot, V., Baert, V., Raemaekers, T., Spittaels, K., Serneels, L., Zheng, H., Saftig, P., De Strooper, B., Klumperman, J. and W. Annaert. 2004. Presenilin 1 deficiency affects the Telencephalin-mediated autophagic degradative pathway in hippocampal neurons. J. Cell Biol. 27;166(7):1041-54.

66. Cerny, J., Feng, Y., Yu, A., Miyake, K., Borgonovo, B., Klumperman, J., Meldolesie, J., McNeil, P.L. and T. Kirchhausen. 2004. The small chemical Vacuolin-1 inhibits Ca++-dependent lysosomal exocytosis but not cell wound healing. EMBO J. 5(9):883-888.

67. Carlton, J., Bujny,. M., Peter, B.J., Oorschot, V.M.J., Rutherford, A., Mellor, H., Klumperman, J., McMahon, H., and P.J. Cullen. 2004. Sorting nexin-1 mediates tubular endosome-to-TGN transport through co-incidence sensing of high curvature membranes and 3-phosphoinositides. Current Biology. 26;14(20):1791-800.

68. Austin, C. D., De Maziere, A. M., Pisacane, P. I., van Dijk, S. M., Eigenbrot, C., Sliwkowski, M. X., Klumperman, J. & Scheller, R. H. 2004. Endocytosis and sorting of ErbB2 and the site of action of cancer therapeutics Trastuzumab and Geldanamycin. Mol Biol Cell 15, 5268-82.

69 Cavalli, V., Kujala, P., Klumperman, J.  and L.S.B. Goldstein. 2005.  Sunday Driver links axonal transport to damage signaling J Cell Biol. 168(5):775-787.

70. Rabouille, C. and J. Klumperman. 2005. The maturing role of COPI vesicles in intra-Golgi transport. Nature Reviews Molecular Cell Biology 6:812-817 (Review on invitation).

71. van Kerkhof P, Lee J, McCormick L, Tetrault E, Lu W, Schoenfish M, Oorschot V, Strous GJ, Klumperman, J, and G. Bu. 2005. Sorting nexin 17 facilitates LRP recycling in the early endosome. EMBO J 24(16):2851-2861.

72. Carlton JG, Bujny MV, Peter BJ, Oorschot VM, Rutherford A, Arkell RS, Klumperman J, McMahon HT, Cullen PJ. 2005. Sorting nexin-2 is associated with tubular elements of the early endosome, but is not essential for retromer-mediated endosome-to-TGN transport. J Cell Sci. 118 (Pt 19):4527-4239.

73. Zeuschner D, Geerts WJC, van Donselaar E, Humbel. B., Slot JW, Koster AJ, and J. Klumperman. 2006. Immuno-electron tomography reveals the existence of free COPII-coated transport carriers. Nature Cell Biol. Epub March 16.

74. Rechards M, Xia W, Oorschot V, van Dijk S, Annaert W, Selkoe DJ, Klumperman J. 2006 Presenilin-1-mediated retention of APP Derivatives in early biosynthetic compartments. Traffic 7(3):354-364.