Z. Josh Huang, Ph.D.
Associate Professor

Cold Spring Harbor Laboratory
Beckman 220
One Bungtown Road
Cold Spring Harbor, NY 11724

Phone: (516) 367-8388
Fax: (516) 367-6805
Email: huangj@cshl.org

1. CURRICULUM VITAE

Education and Employment 
Position                                   Year        Institution
Associate Professor               2004.12 – present                 Cold Spring Harbor Laboratory
Assistant Professor               2000.1 –  2004                      Cold Spring Harbor Laboratory
Postdoctoral fellow                   1994.12 -1999.12                 Dept. of Biology, Massachusetts Inst. Tech.
PhD                                           1989.9  - 1994.8                   Dept.  of Biology, Brandeis University.
MS                                            1986.7 - 1988.12                   Dept. of Zoology. Arizona State University.
 BS                                             1981.9 - 1985.7                     FuDan University, Shanghai, P.R. China 
Professional Training
1994 – 1999 Postdoctoral fellow, Department of Biology and Center for Learning and Memory, Massachusetts Institute of Technology.  Mentor: Susumu Tonegawa.
Cellular and molecular mechanisms of neuronal plasticity in mice.

1989 - 1994:      Ph.D student, Department of Biology, Brandeis University.
Mentor: Michael Rosbash.
Molecular biology, biochemistry, and genetics of circadian rhythms in fruitflies.

1986 - 1988    M.S. student, Department of Zoology, Arizona State University, and the Friday Harbor Laboratories, University of Washington, Seattle.
Neurophysiology of swimming behavior in mollusc and ultrastructural study of molluscan muscle.

1985-1985     Undergraduate thesis with Dr. ZhenTong Mei,  Shanghai Institute of Physiology, Chinese Academy of Science.
Extracellular recording from the prefrontal cortex of the Rhesus Monkey during working memory task.

1990              “Advanced Drosophila  Genetics" summer course, Cold Spring Harbor laboratory.
1997 “Structure, function, and development of the visual system" course, CSHL
1998                            “Imaging structure and function in the nervous system" summer course, CSHL
2005 “DNA microarray" summer course, Cold Spring Harbor laboratory.

Honors and  Awards
2004 – 2007               McKnight Scholar Award in Neuroscience
2003 – 2006               EJLB Foundation Award 
2002 – 2006               Pew Scholar Award
2001                           Cold Spring Harbor Association Award
      2001                           Seraph Foundation Award
2000                           Eppley Foundation Award
1994 - 1997:              Damon-Ruynon Walter-Winchell Postdoctoral Fellowship.
1990:                          Grass Fellowship for "Advanced Drosophila  Genetics" course at the Cold Spring Harbor laboratory.
1987 - 1988:              Regent's Graduate Academic Scholarship, Arizona State University.
1984                           Three-Merit-Student” award, FuDan University. Shanghai, P.R. China

Membership in Professional Societies
1990 --  Society for Neuroscience

Publications
Huang, Z.J., and Satterlie, R.A.(1989). Smooth muscle fiber types and a novel pattern of thick filaments in the wing of the pteropod mollusc Clione limacina. Cell Tiss. Res. 257:405-414.

Huang, Z.J., and Satterlie, R.A. (1990). Neuronal Mechanisms underlying behavioral switching in a pteropod mollusc. J. Comp. Physiol. 166: 875-887.

Lui, X, Yu,Q., Huang, Z.J., Zwiebel, L., Hall, J., and Rosbash, M. (1991). The strength and periodicity of Drosophila melanogaster  circadian rhythms are differentially affected by alterations in period  gene expression. Neuron  6:753-766.

Huang, Z.J., Edery, I., and Rosbash, M. (1993). PAS is a novel dimerization domain shared by the Drosophila period  protein and several transcription factors.  Nature  364:259-262.

Curtin, K., Huang, Z.J.,  and Rosbash, M. (1995). Temporally regulated nuclear entry of the Drosophila Period  protein contributes to the circadian clock.  Neuron  14: 277-286.

Huang, Z.J., Curtin, K., and Rosbash, M. (1995). Period  protein interactions and temperature compensation of a circadian clock in Drosophila melanogaster.  Science 267:1169-1172.

Huang, Z.J., Kirkwood, A., Bear, M.,  Pizzurusso, T., Porciatti, V., Maffei, L., and Tonegawa, S. (1999). BDNF Regulates the Maturation of Inhibition and the Critical Period Plasticity in Mouse Visual Cortex. Cell 98 (6): 739-755.

Hanover, J., Huang, Z.J., Tonegawa, S., and Stryker, M. (1999) BDNF overexpression induces precocious critical period in mouse visual cortex.  Journal of Neuroscience 19(22):RC40).

Huang, Z.J., Yu, W., Lovett, C., and Tonegawa, S. (2002).  A Cre / LoxP recombination activated neuronal marker system in the mouse neocortex and hippocampus. Genesis 32(3):209-217.

Gianfranceschi, L.,  Walls, J., Morales, B., Tonegawa, S., Kirkwood, A. Huang, ZJ., and Maffei, L. (2003) BDNF overexpression rescues the effects of dark rearing on the development of critical period in visual cortex. PNAS.100(21):12486-91.

DiCristo, G., Ango, F., Higashiyama, H., Huang, ZJ., (2004) Subcellular targeting of GABAergic synapses in primary visual cortex in the absence sensory and thalamic input. Nat Neurosci 7(11):1184-1186.

Ango, F., DiCristo, G., Higashiyama, H., Huang, ZJ., (2004) An ankyrin-based subcellular gradient of the immunoglobulin cell adhesion protein neurofascin specifies GABAergic innervation at Purkinje axon initial segment. Cell 119(2):257-272.

Chattopadhayaya, B., DiCristo, G.,  Higashiyama, H., Knott, G., Welker, E., Huang, ZJ., (2004)
experience-and activity- dependent maturation of perisomatic GABAergic innervation in primary visual
cortex during a postnatal critical period. Journal of Neurosci. 24(43):9598-9611.

Pillai-Nair, N., Panicker, A. K.,  Rodriguiz, R.M., Miller, K., Demyanenko, G.P., Huang,  ZJ., Wetsel W.C., and Maness, P.F.  (2005) NCAM-secreting transgenic mice display abnormalities in interneurons and behaviors related to schizophrenia. Journal of Neurosci. 25(18):4659-4671.

Goldberg EM, Watanabe S, Chang SY, Joho RH, Huang ZJ, Leonard CS, Rudy B. (2005)  Specific functions of synaptically-localized potassium channels in synaptic transmission at the neocortical GABAergic fast-spiking cell synapse. Journal of Neurosci. 25(21):5230-5.

Sugino, K., Hempel, C., Miller, M., Hattox, A., Huang, ZJ.,  and Nelson, SB (2006) Molecular taxonomy of major neuronal classes in the mouse forebrain. Nature Neuroscience. 9(1):99-107.

Huang, ZJ., (2006) Subcellular organization of GABAergic synapses: role of ankyrins and L1 cell adhesion molecules. Nature Neuroscience. 9(2):163-6.

Dumitriu, D., Cossart, R., Huang, ZJ., Yuste, R. (2006) Correlation between axonal morphology and synaptic input kinetics in neocortical interneurons. Cerebral Cortex, in press

Huang ZJ. (2006) GABA(B)rReceptor isoforms caught in action at the scene. Neuron. 200618;50(4):521-4.

Chattopadhayaya, B., DiCristo, G.,  Wu, C., Kuhlman, S., Palmiter, R.D., Huang, Z.J., (2006) Regulation of GABAergic synaptic innervation by GAD67-mediated GABA synthesis and signaling. Submitted.

DiCristo, G.,  Chattopadhayaya, B., Wu, C., Kuhlman, S.,  Huang, Z.J., (2006) Activity-dependent PSA-NCAM expression regulates the maturation of perisomatic GABAergic innervation and onset of critical period of ocular dominance plasticity in visual cortex. In preparation.

Kuhlman, S.,  Brody, C., Huang, Z.J., (2006) Maturation of GABAergic inhibition promotes synaptic competition among converging inputs in the visual cortex. In preparation.

Ango, F., Schachner, M., Huang, ZJ., (2006)  Role of CHL1 (CLOSE HOMOLOGUE OF L1) and Bergman glia in the dendrite-targed GABAergic innervation of cerebellar Purkinje neurons. In preparation.

 

2.  RESEARCH SUPPORT

Federal Duration of Grant Direct Costs
RO1-EY13564-01,                                   8/1/01 – 7/31/06   $1,250,000/yr
Principal Investigator: Z. Josh Huang 
Title: GABAergic Circuits Regulate Critical Period in Visual Cortex
Agency: NEI/NIH

NOTEY02001                                           12/1/02 – 11/30/03    $ 372,275
Supplement for Technology Enhancement
Principal Investigator: Hollis Cline, Z. Josh Huang 
Title:  Two-photon imaging of living visual system neurons
Agency: NEI/NIH

R01 NS052784-01,
Principal Investigator: Z. Josh Huang         8/1/05 – 7/31/0 $1,250,000/yr
Agency: NINDS/NIH
Title: Molecular mechanisms of subcellular synapse targeting

Pending RO1
Principal Investigator: Z. Josh Huang 
GABA regulates GABAergic synapse and circuit development.
Submitted Oct 1, 2005

Pending UO1
Principal Investigator: Z. Josh Huang (CSHL), Sacha Nelson (Brandeis Univ)
RFA: “Development of Recombinase-Expressing (“driver”) Mouse Lines for Studying the Nervous System” – Cre expressing mouse lines in the GABAergic system
To be Submitted on Jan 19, 2006

 

International
EJLB Foundation                                         1/1/04 – 1/1/07 CAN$300,000
PI: Z. Josh Huang
Title: L1 Immunoglobulin Cell Adhesion Molecules and Subcellular Synapse Targeting

Private
March of Dimes Birth Defects Foundation  7/1/04 – 6/30/07 $287,408
PI: Z. Josh Huang
Title: Role of L1 Immunoglobulin Cell Adhesion Molecules in the Subcellular Synapse Targeting

The McKnight Endowment Fund for Neuroscience
                                                                     8/1/04-7/30/07 $200,000
PI: Z. Josh Huang
Title: Molecular mechanisms underlying subcellular synapse targeting

Pew Foundation                                         7/1/02 – 6/30/07 $240,000
PI: Z. Josh Huang
Title: Development and plasticity of GABAergic circuits in visual cortex

Whitehall Foundation                                7/1/01 – 6/30/04 $225,000
PI: Z. Josh Huang
Title: GABAergic circuits regulate critical period plasticity in visual cortex

Postdoc Fellowships
NARSAD Foundation (2) 04 –06, 05-07                    $60,000 x 2
Human Frontier  02  - 05                              $108,000
EMBO 02 – 04                              $73,648
NRSA (2) 02 – 05, 05-08                   $130,234 x 2
Cold Spring Harbor Association 01                                      $35,000
Cold Spring Harbor Association 04                                      $35,000

3. STUDENTS AND TRAINEES

UnderGraduate Research Program (URP)
 
Name Year Institution   
Joe Markson 2001 Harvard   
Jonathan Hertz 2002 MIT   
Daniel Jones 2003 Pomona College   
Nick Wall 2004 Caltech 

Current Trainees
 
Name Post/Pre Date Institution Grant  Current Position   
Hiroki Taniguchi Postdoc 11/05- Univ. of Osaka,
Japan     
Hong Yang Postdoc 7/03- Harvard Medical School CSH Association postdoc fellowship;
NRSA fellowship    
Sandra Kuhlman Postdoc 8/01- Univ. of Kentucky,
Med. Sch. NRSA postdoctoral fellowship    
Anirban Paul Postdoc 12/05 Howard Univ.     
Caizhi Wu Postdoc 12/02- Shandong
Medical School/Weismann Inst, Isreal     
Xiaoyun Wu Predoc 7/05- FuDan Univ.
Shanghai, China   

Past Trainees
 
Name Post/Pre Date Institution Grant  Current Position   
Graziella Di Cristo Postdoc 4/01-3/06 Scuola Normale Superior, Pisa, Italy EMBO Long term fellow; NARSAD postdoc fellowship Assistant Professor
Dept. of Physiology
Univ. Montreal, Canada   
Fabrice Ango Postdoc 1/02-11/05 Center CNRS-INSERM HFSP Postdoctoral Fellowship; NARSAD postdoc fellowship Assistant Professor
Inst of Functional Genomics,
CNRS, Montpellier, France   
Bidisha Chattopadhyaya Predoc 7/00-12/05   Postdoc Fellow, Max Planck Inst. Medicine, Heidelberg 

4.  INVITED DEPARTMENTAL SEMINARS
Date Department, Institution, City, State (partial list)
11/2/06 Dept of Neuroscience, University of Massachusetts School of Medicine, Worcester, MA
10/19/06 Dept of Neuroscience, Albert Einstein School of Medicine, New York
4/5/06 Dept of Cell Biology, Yale University Medical School, New Haven, CT
3/15/06 Dept of Neuroscience, Georgetown University School of Medicine, Washington DC
3/1/06 Program in Neuroscience, Cornell University Medical School, New York City
2/24/06 Dept of Neuroscience, Baylor College of Medicine, Houston, TX
1/25/06 Program in Neuroscience, University of Vanderbilt, Tennasee
1/12/06 Program in Neuroscience, University of California San Francisco, CA

11/29/05 Dept of Physiology, Columbia University College of P&S, New York City
11/7/05 Skirball Institute,  New York University Medical School, New York City
4/27/05 Broad Institute of MIT and Harvard, Cambridge, MA
4/20/05  Dept of Neuroscience Mount Sinai School of Medicine, NYC, NY
2/28/05 Dept. of Physiology, University of California San Francisco, CA
2/24/05 Neuroscience Program, Stanford University, Palo Alto, CA
2/4/05 Division of Cellular and Systems Neurobiology, Max-Planck-Institut fuer Neurobiologie, Munchen-Martinsried, GermanyMunich
2/3/05 Dept Neurobiology, Max-Planck-Institute for Medical ResearchMax Plank Institute, Heidelberg, Germany
2/2/05 Zentrum für Molekulare Neurobiologie, University of Hamburg, Germany
1/31/05 Max-Delbrueck-Centrum fuer Molekulare Medizin, Berlin, Germany

11/30/04 Division of Neuroscience, Children’s Hospital, Harvard Medical School, Boston, MA
11/8/04 Dept of Neuroscience and Psychiatry, University of Pittsburgh Medical School
10/8/04 Picower Center, Dept. of Brain and Cognitive Science, MIT, Cambridge, MA
5/15/04 Institute of Neurophysiology, Scola Normale, Pisa, Italy

11/18/03 Dept. of Biology, Brandeis University, Waltham, MA
9/24/03 Neuroscience Institute, EPFL, Lausanne, Switzerland
9/22/03 Dept. of Biological and Cellular Morphology, University of Lausanne, Switzerland
9/19/03 Fredrik Meiser Institute, Basel, Switzerland
5/1/03 Brain Research Inst. & Neuroscience Program, UCLA, Los Angeles, CA
4/9/03 Dept. of Neurobiology, Duke University Medical School
4/8/03 Dept. of Cell and Molecular Physiology, Neuroscience Center, Univ. Of North Carolina, NA
2/20/03 Dept. of Biology, Neuroscience Program, Univ. of Maryland, College Park, MD

12/4/02 Neurobiology Group, Dept. of Biological Sciences, Columbia University
8/16/02 Chinese Institute of Neuroscience, Shanghai, China
4/3/02 Dept. of Physiology, Univ. of West Virginia School of Medicine. Morgantown, WV.

5.  INVITED CONFERENCES
Date Title, Location
3/18/07 Expanding the Genetic Tool-kit in Mouse, Howard Hughes Medical Institute
8/20/06 Gordon Conference on Neural Development, Rhode Island
6/11/06 Gordon Conference on Molecular and Cellular Neurobiology, Hong Kang, China
12/11/05 Symposium speaker, American College of Neuropsychopharmacology, Hawaii
9/12/05  Workshop on “Synapse Function and Abnormal Development in Children”, Fondation des Treilles, France
10/30/05 Organizer: The GABAergic System, Banbury Conference, Cold Spring Harbor
7/5/05 Workshop on Schizophrenia.  Banbury, Cold Spring Harbor
12/15/04    Interneurons and Cortical Function, Whistler, British Columbia, Canada
10/15/04 EJLB Conference, Hovery Manor, North Hatley, Quebec, Canada
9/15/04 The Multiple Facets of GABAergic Synapses, La Ciotat, France
3/25/04 Cortical Columns, Cosney Workshop, Cold Spring Harbor
9/26/03 FMI conference: Neural Circuit, Ascona, Switzerland
2/2/03 Winter Brain Research Conference, Snowbird, Colorado
8/10/02 International Conference in Neuroscience, Chengdu, Sichuan, China
7/13/02 Visual Cortical Plasticity, European Society of Neuroscience, Paris, France
 
Conference Organizer
12/7-/06  The GABAergic System, Cold Spring Harbor
 with Drs. Gyory Buzsaki, Henry Markram, Roger Nicoll
10/30/05 The GABAergic System, Banbury Conference, Cold Spring Harbor
 with Dr. Gyory Buzsaki
7/05           Workshop on Schizophrenia and Related Disorders, Banbury, Cold Spring Harbor
 with Dr. Jeff Lieberman, Paul Harrison, Daniel Weinberger, John Lisman
6/14/04      Mini-symposia on Schizophrenia, Cold Spring Harb or, with James D. Watson

6. COURSE LECTURES
Date Title
8/18/03 Imaging Structure and Function of the Nervous System, CSHL
8/14/02  Imaging Structure and Function of the Nervous System, CSHL
6/15/02 Developmental Neurobiology, CSHL
  
7.  PROFESSIONAL SERVICE

Grant Review Committees
                         MDCN7 Study Section, NIH, Washington DC.
 
Manuscript Reviews
 Science 
 Neuron
 Journal Neuroscience 
 Genes & Development 
 Journal of Cell Biology
 Journal Neuroscience Method  
 Molecular and Cellular Neuroscience   
Genesis  
European Journal of Neuroscience 

8.  CSHL SERVICE

Presentations
Date Title, Location
3/5/04       The GABAergic Circuit Development Hypothesis of Schizopbrenia. Pulling, Beckman
 
Teaching
Date   Title
10//04 Cell Junctions; Cell biology – Signaling; Waston School of Biological Sciences, CSHL
10//05 Cell Junctions; Cell biology – Signaling; Waston School of Biological Sciences, CSHL
12/05 Scientific Reasoning and Logic – Grant Module Waston School of Biological Sciences, CSHL

Committees
Date Title
1/01-- Committee on Animal Care (IACUC), Chair since 1/05
 Thesis Committee: Brian Chen, Jonathan Kui, Wei wei (Chair)