Phosphate Buffer

Phosphate Buffer (0.2M PB, pH 7.4):

       Na2HPO4 -------------------------------------- 21.8 g

       NaH2PO4 -------------------------------------- 6.4 g

       Distilled water --------------------------------- 1000 ml

       Mix to dissolve and adjust pH to 7.4

       Store this solution at room temperature.

Phosphate Buffer (0.1M PB, pH 7.4):

       Na2HPO4 ------------------------------------- 10.9 g

       NaH2PO4 ------------------------------------- 3.2 g

       Distilled water -------------------------------- 1000 ml

       Mix to dissolve and adjust pH to 7.4

       Store this solution at room temperature.