Description
DRD2 TaqI "A" Polymorphism

Procedure
Taq I "A" Located >10 kb downstream from exon 8

TEMPLATE: Genomic DNA 100-200 ng/reaction volume of 25 µl.

Primer 1: 5014 MOD: '5- CCT TCC TGA GTG TCA TCA AC 100 ng/reaction
Primer 2: TAQACK1: '5- ACG GCT CCT TGC CCT CTA G 100 ng/reaction

Buffer: Final Concentration 2.25 mM MgCl2, 50 mM KCl, 10 mM Tris HCI pH 8.4

Taq DNA Polymerase: 0.5 u/reaction volume of 25 µl
(Perkin-Elmer Amplitaq DNA polymerase 5u/µl, Cat No. N808-0145)

PCR Cycling Profile: 94oC (5')
94oC (30"), 60oC (30"), 72 (1.0') × 30 cycles

Product size: Taq I
236 bp ——–> 124 bp + 112 bp (in homozygote present)

Grandy, D.K., Y. Zhang, and O. Civelli, 1993. PCR detection of the TaqI A RFLP at the DRD2 locus. Hum. Mol. Genet. 2:2197.

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