Saturday, May 15, 2004

Description
NGFR TaqI(a) Polymorphism

Procedure
TaqI(a)

(7.18 KB DWST OF EXON 6)

Template: Genomic DNA 100-200 ng/reaction

Primer 1. NGFRTQAF1 5' GAC ACT GGT TAT ACT CTA GTC C 3' 100 ng/reaction
Primer 2. NGFRTQAR1 5' CAG TGG CTT GCA GAT GAT TC 3' 100 ng/reaction

dNTPs: Final Concentration 200 M each

Buffer: Final Concentration 1.5 mM MgCl2, 50 mM KCl, 10 mM Tris HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
(Perkin-Elmer Amplitaq DNA polymerase 5 U/l, Cat. No. N808-0145)

PCR Cycling Profile: 94C, 5 min
94C (30"), 60C (30"), 72C (30") x 30 cycles
72C (7')

Product size: Taq I
423 --------------> 82 and 341 bp in heterozygote


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