Saturday, May 15, 2004

Description
PAH XmnI Polymorphism

Procedure
XmnI; LOCATED ABOUT 2 KB 3' OF THE MSPI POLYMORPHISM IN INTRON 8

Template: Genomic DNA 100-200 ng/reaction volume of 25 l

Primer 1. PAHXMN1 5 -CTG TAC TTG TAA GAT GCA GC-3' 100 ng/reaction
Primer 2. PAHXMN2 5 -ACT GTC CCA AGC AAT CAA AG -3' 100 ng/reaction

dNTPs: Final Concentration 200 M each

Buffer: Final Concentration 2.25 mM MgC12, 50 mM KCl, 10 mM Tris HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
(Perkin-Elmer Amplitaq DNA polymerase 5 U/l, Cat. No. N808-0145)

PCR Cycling Profile: 95C, 5'
95C (30"), 58C (30"), 72C (30") x 30 cycles

Product size: XmnI
205 bp ----------->110 bp and 95 bp (+/+ individual)



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