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[RNA] Deprotection of Synthetic RNA 日期：2005-07-18 00:00:00 点击：245 好评：0
Monday, October 20, 2003 Description This protocol describes the desilylation or 2' deprotection of synthetic RNA. Procedure 1. After synthesis on the 1 M scale, take the column apart with pliers and deposit the resin in a glass deprotecti...
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[RNA] RNase Protection and Immunoprecipitation of Nuclear Extracts 日期：2005-07-18 00:00:00 点击：177 好评：0
Monday, October 20, 2003 Description RNase Protection and Immunoprecipitation of Nuclear Extracts Procedure A. Prepare Beads (for 15 reactions) 1. Resuspend 60 mg of Protein A-Sepharose CL-4B or 375 l Protein G-Sepharose in 7.5 ml of NET2...
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[RNA] RNase Protection and Immunoprecipitation of Nuclear Extracts 日期：2005-07-18 00:00:00 点击：140 好评：0
Sunday, October 19, 2003 Description RNase Protection and Immunoprecipitation of Nuclear Extracts Procedure A. Prepare Beads (for 15 reactions) 1. Resuspend 60 mg of Protein A-Sepharose CL-4B or 375 l Protein G-Sepharose in 7.5 ml of NET2...
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[RNA] Native Gel Electrophoresis of RNA-Protein Complexes 日期：2005-07-18 00:00:00 点击：317 好评：0
Sunday, October 19, 2003 Description This protocol is optimized to resolve complexes formed on short (50 to 70 nucleotide) RNA oligonucleotides. Incubation conditions, percentage of Acrylamide, and length of separation in the gel should be...
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[RNA] EXTRACTION AND PURIFICATION OF TOTAL RNA USING TRIzol 日期：2005-07-18 00:00:00 点击：352 好评：0
Friday, October 17, 2003 Description RNA Extraction using Trizol reagent Procedure 1. Homogenization for Cell Suspensions a. Place 1 ml aliquots of the cell suspension in sterile RNase free 1.5 ml microcentrifuge tubes. b. Centrifuge for 1...
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[RNA] Total cellular RNA 日期：2005-07-18 00:00:00 点击：341 好评：0
Friday, October 17, 2003 Description Isolation of total cellular RNA using 4M guanidinium isothiocyanate lysis buffer and caesium chloride ultracentrifugation Large scale isolation of total cellular RNA from adult organs Whole organs are h...
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[RNA] RNA from tissues. 日期：2005-07-18 00:00:00 点击：251 好评：0
Friday, October 17, 2003 Description Procedure for extraction of RNA from tissues. Procedure 1.Place RNAlater stabilized tissue is to be used, in DEPC treated glass petridish for cutting, and cut it. Place it into a suitably sized vessel f...
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[PCR] DNA precipitation 日期：2005-07-18 00:00:00 点击：375 好评：0
Monday, June 07, 2004 Description This protocol describes a method to precipitate DNA in order to concentrate the DNA fragment or plasmid fo further downstream processes. Procedure 1 Extract DNA fragment following PCR reaction using a comm...
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[PCR] Mini prep and restriction digest conformation 日期：2005-07-18 00:00:00 点击：361 好评：0
Monday, June 07, 2004 Description This precedure describes a method for preparation of plasmid DNA and confirmation by restriction endonuclease digestion. See also protocol for cloning of DNA fragments. Procedure 1 Grow bacterial colonies...
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[PCR] Cloning of DNA fragments 日期：2005-07-18 00:00:00 点击：334 好评：0
Monday, June 07, 2004 Description This protocol describes the various steps involved in clonig a DNA fragment into a vector fro production of a plasmid via bacterial cells. This cincudes teh cDNA synthesis, PCR reaction, ligation reaction...