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[PCR] Differential Display PCR 日期：2005-07-18 00:00:00 点击：370 好评：0
Friday, June 04, 2004 Description To identify and characterize those genes that are differentially expressed in order to understand the molecular nature of disease state and subsequently, to devise rational therapies. Differential display...
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[PCR] RAPD ( Randomly Amplified Polymorphic DNA) 日期：2005-07-18 00:00:00 点击：198 好评：0
Friday, June 04, 2004 Description RAPD ( Randomly Amplified Polymorphic DNA) analysis with 'P. infestans' Procedure Reaction conditions: 25 ul per reaction sterile di water 16 ul 1O X buffer 2.5 ul 100 mM KCI 200 mM Tris-HCI (pH 8.8) 100 m...
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[PCR] PCR of GC rich templates 日期：2005-07-18 00:00:00 点击：402 好评：0
Sunday, February 01, 2004 Description High GC content can prohibit the PCR reaction due to improper denaturing of the template or annealing of the primers. This minor spin on a common protocol is simple but works incredibly well. Procedure...
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[PCR] Tail PCR 日期：2005-07-18 00:00:00 点击：600 好评：0
Thursday, December 04, 2003 Description Tail PCR Procedure Amplification of flanking sequences To determine flanking sequences of RDA subtraction products in the genome, a two-step PCR reaction technique can be applied using a biotinylated...
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[PCR] OLIGO PURIFICATION on acrylamide gels 日期：2005-07-18 00:00:00 点击：314 好评：0
Friday, November 21, 2003 Description OLIGO PURIFICATION on acrylamide gels Procedure GEL PURIFICATION OF OLIGONUCLEOTIDES 1. Quantitate crude oligo solution via UV spectrophotometry. Assume 1.0 A260 unit is equal to 33 ug/ml. Dry down 100...
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[PCR] PRIMER LABELLING FOR PRIMER EXTENSION ASSAY and PRIMER EXTEN 日期：2005-07-18 00:00:00 点击：368 好评：0
Friday, November 21, 2003 Description PRIMER LABELLING FOR PRIMER EXTENSION ASSAY and PRIMER EXTENSION Procedure Mix in a 20ul final volume: 2ul 10x kinase buffer 1ul oligonucleotide primer (5pmoles/ul) 200uCi 32P-gATP (crude, ICN, 7000Ci/...
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[PCR] DNA-se I FOOTPRINTING 日期：2005-07-18 00:00:00 点击：335 好评：0
Friday, November 21, 2003 Description This technique exploits the fact that a protein bound to a specific DNA sequence will interfere with the digestion of that region by the endonuclease DNAaseI. In summary, an end-labelled DNA probe is i...
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[PCR] Nested Deletions using exonuclease-III and mung bean nucleas 日期：2005-07-18 00:00:00 点击：203 好评：0
Friday, November 21, 2003 Description Nested Deletions using exonuclease-III and mung bean nuclease Procedure ExoIII has a 3' to 5' exonuclease activity which must initiate on blunt ended DNA or DNA with a 5' overhang. 1. A nested series o...
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[PCR] Nested Deletions using exonuclease-III and mung bean nucleas 日期：2005-07-18 00:00:00 点击：307 好评：0
Friday, November 21, 2003 Description Nested Deletions using exonuclease-III and mung bean nuclease Procedure ExoIII has a 3' to 5' exonuclease activity which must initiate on blunt ended DNA or DNA with a 5' overhang. 1. A nested series o...
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[PCR] In vitro mutagenesis using Altered Sites 日期：2005-07-18 00:00:00 点击：293 好评：0
Friday, November 21, 2003 Description In vitro mutagenesis using Altered Sites Procedure A. Isolation of Single Stranded DNA 1. Transform plasmid to be mutagenised into JM109. JM109 is endA1, recA1, gyrA96, thi, hsdR17(rk-, mk+), relA1, su...