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[Immunology] Flow Cytometric Analysis Of Bcl Family members 日期：2005-07-18 00:00:00 点击：205 好评：0
Friday, October 17, 2003 Description Cell Fixation, staining and flow cytometric analysis Procedure Cells (106) were washed twice in FACS buffer (phosphate buffered saline PBS pH 7.4 containing 2% fetal calf serum(FCS) and 0.02% NaN3) and...
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[Immunology] CD3/ CD4 /CD8 T cell subset counts 日期：2005-07-18 00:00:00 点击：290 好评：0
Friday, October 17, 2003 Description Procedure for CD3/ CD4 /CD8 T cell subset counts using CD3-FITC / CD4-PE / CD8-PECy5 antibody Procedure 1.One 5 ml round bottom tube (Falcon) is taken per blood sample to be analyzed. The tube is labele...
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[Immunology] DNA Staining for Cell Cycle Analysis By flow Cytometry 日期：2005-07-18 00:00:00 点击：183 好评：0
Thursday, October 16, 2003 Description This method of DNA staining utilizes ethanol to fix the cells and permeabilize the membrane, which allows the dye (Propidium Iodide) to enter the cells. Propidium Iodide (PI) is a DNA-binding fluoroch...
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[Immunology] Direct Immunofluorescence Labeling 日期：2005-07-18 00:00:00 点击：225 好评：0
Thursday, October 16, 2003 Description This is the method for direct immunofluorescence labeling; that is, the antibodies have the fluorescent dye attached. Direct labeling is simpler and quicker than indirect labeling. Procedure Adjust th...
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[Immunology] IMMUNOCYTOCHEMISTRY 日期：2005-07-18 00:00:00 点击：190 好评：0
Thursday, October 16, 2003 Description Procedures for cell plating, fixing, antibody incubation and mounting Procedure A. Plating: To sterilize glass coverslips, dip in ethanol and flame. We use 22x22x1 mm3 coverslips and put them in 6-wel...
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[General Lab] Quantitative Determination of Sulfhydryl (-SH) Groups in Pep 日期：2005-07-18 00:00:00 点击：283 好评：0
Tuesday, March 01, 2005 Description This is the standard Ellmans Test for the determination of free thiols. (Ref. 1) It works well for small peptides and proteins synthesized using standard solid phase synthetic methods. Peptides from thes...
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[General Lab] Preparation of competent cells. 日期：2005-07-18 00:00:00 点击：487 好评：0
Monday, June 07, 2004 Description This rubidium chloride protocol gives better transformation efficiencies than calcium chloride procedures for most strains. Procedure 1) Innoculate a single colony from an LB plate in 2.5 ml of LB medium....
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[General Lab] Making Mg++ /Ca++ competent E.Coli BL21 cells 日期：2005-07-18 00:00:00 点击：318 好评：2
Wednesday, June 02, 2004 Description This protocol explains how to prepare Magnesium/Calcium competent cells for transformation with DNA. It is described using BL21 cells but other E. Coli cells could be used. Procedure 1: Innoculate a sin...
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[General Lab] Rapid disruption of bacterial colonies to test the size of p 日期：2005-07-18 00:00:00 点击：246 好评：0
Wednesday, June 02, 2004 Description This method can be use to quickly identify if the correct plasmid (identified here by size) has been transformed into a bacterial cell (generally E. Coli). Procedure 1: Grow bacterial colonies on rich a...
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[General Lab] Post-Embedding Immunogold Method 日期：2005-07-18 00:00:00 点击：259 好评：0
Wednesday, May 05, 2004 Description Post-Embedding Immunogold Method Procedure L.R. white sections are collected on gold Veco grids. If blocking is necessary for nonspecific labeling, buffer is supplemented with normal goat serum (2 - 10%)...