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  • [Course] Spiking Neuron Models 日期:2007-02-13 02:08:48 点击:427 好评:0

    Spiking Neuron Models Single Neurons, Populations, Plasticity Wulfram Gerstner and Werner M. Kistler Cambridge University Press (August 2002) paperback: ISBN 0 521 89079 9 : 40 USD hardcover: ISBN 0 521 81384 0 : 90 USD Order directly from...

  • [Course] WEBVISION The Organization of the R 日期:2007-01-31 06:53:54 点击:657 好评:0

    Foundations Introduction Gross Anatomy of the Eye Simple Anatomy of the Retina Overview Central and peripheral retina compared Muller Glial Cells Foveal Structure Macula Lutea Blood supply to the retina Degenerative diseases of the human r...

  • [PCR] PCR Transformation 日期:2006-08-17 06:28:24 点击:433 好评:0

    PCR Transformation Grow cells to an OD600 0.7-2.5 (25ml culture) Spin cells 4500 rpm for 2 min Resuspend in 50 ml 0.1M LiOAc Repeat step 2 and 3 Resuspend in 0.1M LiOAc (culture volume x OD/100) Add 1/9 volume of 10 mg/ml ssDNA that has be...

  • [RNA] Isolation of total cytoplasmic RNA  日期:2005-07-18 00:00:00 点击:531 好评:0

    Description Isolation of total cytoplasmic RNA using LiCl-Urea extraction and PK digestion – Auffray and Rougeon modified method RNA extraction suitable for RNases rich tissues Procedure Sacrifice animals by cervical dislocation. Dissect...

  • [RNA] RNA denaturing gel - BTPE 日期:2005-07-18 00:00:00 点击:515 好评:0

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  • [RNA] RNAi in HeLa cells 日期:2005-07-18 00:00:00 点击:577 好评:0

    Sunday, February 08, 2004 Description This is a popular procedure, in fact, it could be the most popular mammalian genetic technique out there right now. The only issue is that most labs seem to do it differently with variable success. Her...

  • [RNA] Ribonuclease Protection Assay 日期:2005-07-18 00:00:00 点击:446 好评:0

    Wednesday, November 19, 2003 Description Most RNase Protection protocols require an overnight hyb with numerous subsequent clean-up steps. This method requires a maximum hyb of four hours, and the clean-up steps are the barest minimum, yet...

  • [RNA] RNA synthesis from plasmid-borne sequences 日期:2005-07-18 00:00:00 点击:390 好评:0

    Tuesday, November 18, 2003 Description In vitro RNA synthesis from plasmid-borne sequences under the control of T phage promoters Procedure 1) Linearize the plasmid at, or near, the terminus of the insert cDNA with a suitable restriction e...

  • [RNA] Yeast Chromatin Immunoprecipitation 日期:2005-07-18 00:00:00 点击:452 好评:0

    Tuesday, November 18, 2003 Description Yeast Chromatin Immunoprecipitation Procedure Day 1 1. Start 5 ml o/n cultures of strains to be tested Day 2 1. Inoculate 50 ml of the desired media with a volume of a saturated o/n culture and grow o...

  • [RNA] Single Stranded dut ung DNA Prep 日期:2005-07-18 00:00:00 点击:331 好评:0

    Tuesday, November 18, 2003 Description Single Stranded dut ung DNA Prep Procedure Transform the plasmid to be mutagenized into strain CJ236 (dut- ung-). Patch several transformants to YT amp +chloramphenicol (30 micrograms/ml; make fresh c...

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