我们热爱生命科学!-生物行
当前位置: 主页 > English > Technology > Methods
  • [RNA] Isogen RNA Extraction Protocol 日期:2005-07-18 00:00:00 点击:997 好评:0

    Monday, November 10, 2003 Description Isogen RNA Extraction Protocol Procedure This protocol is for 200mg of leaf tissue Add 0.25ml of ddH2O and 0.75ml of Isogen-LS to eppendorf tube (one per sample) Vortex to mix Hold mortar in liquid N2...

  • [RNA] Methods for Plant RNA Isolation 日期:2005-07-18 00:00:00 点击:411 好评:0

    Friday, November 07, 2003 Description Methods for Plant RNA Isolation Procedure AMES/Chloroform extraction in our lab to extract total RNA from potato leaves for viroid analysis. The protocol is as follows: AMES Buffer (for 200mL):11.7g Na...

  • [RNA] In Vitro Transcription Assay (Run-off&nb 日期:2005-07-18 00:00:00 点击:568 好评:0

    Description With this protocol, transcripts that were initiated from specific genes by RNA polymerases prior to permeabilization can be measured. Instead of a nuclear extract, permeabilized cells are used. Procedure A. Permeabilization of...

  • [RNA] RNase Protection and Immunoprecipitation of Nuclear Extracts 日期:2005-07-18 00:00:00 点击:250 好评:0

    Tuesday, October 21, 2003 Description RNase Protection and Immunoprecipitation of Nuclear Extracts Procedure A. Prepare Beads (for 15 reactions) 1. Resuspend 60 mg of Protein A-Sepharose CL-4B or 375 l Protein G-Sepharose in 7.5 ml of NET2...

  • [RNA] Complete RNase T1 Digest 日期:2005-07-18 00:00:00 点击:330 好评:0

    Tuesday, October 21, 2003 Description RNase T1 is an endonuclease that specifically cleaves 3' of G residues in RNA. This protocol uses RNase T1 for RNA mapping studies. Procedure 1. Dry the RNA sample in either a vacuum centrifuge or a ly...

  • [RNA] RNase Protection Assay 日期:2005-07-18 00:00:00 点击:286 好评:0

    Description This protocol can be used to quantitate an mRNA of interest if the RNA probe is present in molar excess over the target mRNA. A labeled RNA transcript is hybridized to an mRNA population and any non-hybridized (single stranded)...

  • [RNA] RNA Primer Extension 日期:2005-07-18 00:00:00 点击:283 好评:0

    Monday, October 20, 2003 Description RNA Primer Extension Procedure 1. Precipitate the RNA with 5' end-labeled primer in 0.3 M Sodium Acetate with 2.5 volumes of 100% Ethanol (See Hint #1). 2. Mix well by inversion, microcentrifuge to pell...

  • [RNA] Reverse Transcriptase Assay Using DEAE-Cellulose 日期:2005-07-18 00:00:00 点击:246 好评:0

    Monday, October 20, 2003 Description Reverse Transcriptase Assay Using DEAE-Cellulose Procedure 1. Take 10 l of medium from the wells of a 96-well microtiter plate containing a confluent cell culture of the desired cell line and put it int...

  • [RNA] Deprotection of Synthetic RNA 日期:2005-07-18 00:00:00 点击:245 好评:0

    Monday, October 20, 2003 Description This protocol describes the desilylation or 2' deprotection of synthetic RNA. Procedure 1. After synthesis on the 1 M scale, take the column apart with pliers and deposit the resin in a glass deprotecti...

  • [RNA] RNase Protection and Immunoprecipitation of Nuclear Extracts 日期:2005-07-18 00:00:00 点击:177 好评:0

    Monday, October 20, 2003 Description RNase Protection and Immunoprecipitation of Nuclear Extracts Procedure A. Prepare Beads (for 15 reactions) 1. Resuspend 60 mg of Protein A-Sepharose CL-4B or 375 l Protein G-Sepharose in 7.5 ml of NET2...

栏目列表
推荐内容