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  • [Cell Culture] Cell Clonogenic Survival Assay 日期:2006-07-24 14:10:21 点击:160 好评:0

    Wednesday, May 04, 2005 Description Allows one to test the capability of adherent cells to survive and replicate following insult with chemicals or radiation. Procedure 1. Grow the cells in regular media to 70% confluence and treat the cel...

  • [Cell Culture] Protein extraction for SDS-PAGE from human monocytes 日期:2006-07-24 14:10:19 点击:46 好评:0

    Wednesday, May 18, 2005 Description Protocol for rapid extraction of undegraded proteins from cultured human monocytes for the purpose of immunoblotting Procedure Isolate PBMCs from Buffy Coats as usual, and select CD14+ monocytes using an...

  • [Cell Culture] Primary neuron viability 日期:2006-07-24 14:10:18 点击:31 好评:0

    Wednesday, June 01, 2005 Description Fluorescence based asssay for establishing neuronal viability in cell culture monolayers. Procedure Monolayer cultures of rat embryonic cortical neurons were prepared and plated at a density of 2-5 x 10...

  • [Cell Culture] Dorsal Root Ganglion Neuron Culture from Adult Rats 日期:2006-07-24 14:10:17 点击:43 好评:0

    Sunday, June 12, 2005 Description Procedure for culturing and maintaining Dorsal Root Ganglion (DRG) neurons from adult rats. Procedure Preparing Culture Surface -Collagen coat cover slips (if using) or cell culture dishes: evenly spread 2...

  • [Cell Culture] Differentiation of 3T3-L1 fibroblasts to adipocytes 日期:2006-07-24 14:10:16 点击:36 好评:0

    Tuesday, June 14, 2005 Description We maintain the stock of 3T3-L1 cells in 150 mm tissue culture dishes in DMEM (Gibco catalog no. 11995-065) supplemented with 10% Fetal Bovine Serum, 100 units/ml of Penicillin and 100 礸/mL of Streptomyc...

  • [Animal] A Simple Method for Collecting Embr 日期:2006-07-07 15:21:15 点击:286 好评:0

    A Simple Method for Collecting Embryos from Xenopus tropicalis By Vladimir S. Apekin /Sive Lab Introduction The small size of the X. tropicalis makes the use of the traditional X. laevis methods of embryo collection very difficult. Therefo...

  • [Animal] Anatomy of the 24, 48, 72 and& 日期:2006-07-07 14:38:03 点击:267 好评:0

    Anatomy of the 24, 48, 72 and 120 hours Zebrafish ( Danio rerio ) Embryo This collection of sections through zebrafish embryos at four different stages of development is thought to provide some help to understand how the zebrafish embryo l...

  • [Animal] THE ZEBRAFISH BOOK 日期:2006-07-07 14:00:21 点击:1378 好评:0

    A guide for the laboratory use of zebrafish Danio...

  • [Electrophysiology] Axon Patch Clamp Amplifiers for Ele 日期:2006-06-20 20:30:10 点击:1643 好评:0

    MULTICLAMP 700B Current and Patch Clamp Amplifier Computer-controlled microelectrode amplifier for resistive-feedback patch clamp, high speed current lamp, voltammetery, amperometry ion selective measurement and bilayer recording. Supplied...

  • [Electrophysiology] Electrophysiological recordings from the  日期:2006-06-20 20:24:25 点击:414 好评:0

    Electrophysiological recordings from the pharynx* Alan Cook, Christopher J. Franks and Lindy Holden-Dye § School of Biological Sciences, University of Southampton, SO16 7PX, UK -------------------------------------------------------------...

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