Monday, October 27, 2003 Description JNK/SAP Kinase Assay Procedure A. Stimulation and Harvesting of Cells 1. Grow cells to confluence in 100 mm culture dishes. 2. After cells reach confluence, grow in serum-free media for up to 48 hours....
Monday, October 27, 2003 Description This protocol uses mitogen-activated protein/ERK kinase (MEK) to activate the extracellular-signal-regulated (Erk) mitogen-activated protein (MAP) kinases upon agonist binding to receptors. Procedure A....
Description This protocol describes a semi-quantitative in vitro assay to detect telomerase activity. Any active telomerase present in a cell extract from detergent-lysed cells will add a variable number of telomeric repeats (TTAGGG) to th...
Monday, October 27, 2003 Description The purpose of this fluorescent method is to simultaneously measure apoptotic cells, necrotic cells, normal (non-apoptotic/non-necrotic) cells, and apoptotic cells that have undergone secondary necrosis...
Monday, October 27, 2003 Description CPP32 Activity Assay Using CPP32 Fluorogenic Substrate Procedure . Determine the protein concentration of the sample to be analyzed (see protocol on Quantification of Protein Samples). For each reaction...
Description The Under-Agarose assay is a useful method for observing the response of a cell population to one or more chemoattractant sources. The behavior of individual migrating cells can be studied by modifying the assay for video micro...
Saturday, October 18, 2003 Description Bioassay for IFN-gamma Procedure 1) Prepare L929 or A549 cell suspension at a density of 3.5 x 105/ml in assay medium. Add 100 l/well to the 96-well plate and incubate overnight at 37 degrees C, 5% CO...
Saturday, October 18, 2003 Description This protocol is for use with the D cyclins and employs 488 nm argon laser excitation of propidium iodide and 630 nm NeNe or diode laser excitation of the fluorochrome Cy5 to detect cell cycle-specifi...
Friday, October 17, 2003 Description Cell Fixation, staining and flow cytometric analysis Procedure Cells (106) were washed twice in FACS buffer (phosphate buffered saline PBS pH 7.4 containing 2% fetal calf serum(FCS) and 0.02% NaN3) and...
Friday, October 17, 2003 Description Procedure for CD3/ CD4 /CD8 T cell subset counts using CD3-FITC / CD4-PE / CD8-PECy5 antibody Procedure 1.One 5 ml round bottom tube (Falcon) is taken per blood sample to be analyzed. The tube is labele...