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  • [分子生物学] 别具风格,种类凡多,而且满足大众实验室需求的[高分辨率,低熔点及高标准熔点]琼脂糖 日期:2006-08-26 15:44:26 点击:1014 好评:2

    琼脂糖产品( Agarose Products ) 生产商:美国 Ⅰ . 属分子生物学级别的琼脂糖: SeaKem LE , SeaPlaque 和 NuSieve 3:1 。 属遗传学技术级别的琼脂糖: SeaKem GTG , SeaPlaque GTG , NuSieve GTG , Seakem Gold 和 Incert 。 Ⅱ . 琼脂糖分类,如下...

  • [分子生物学] Protocol for DNA Sequencing via PCR 日期:2006-03-25 19:28:50 点击:688 好评:0

    ProtocolforDNASequencingviaPCR CleanPCRproduct 1.Add100μlPCRproductstoa1.5mlmicrocentrifugetube.Add1000μlResi nandmixbyvortex3minutes. 2.Takeonedisposablesyringe,removetheplungerandplugonecolumn(forDN Apurification)onthetipofsyringe.Trans...

  • [分子生物学] Investigation of Intracellular Zinc Stor 日期:2006-03-20 17:18:53 点击:507 好评:0

    Zinc is an essential trace element in all forms of life. Despite its critical importance in maintaining such basic functions as proliferation and cell growth, little is known about how zinc is acquired, stored, and utilized by the cell. Re...

  • [分子生物学] NHGRI将测序8种新的哺乳动物序列 日期:2006-03-19 13:42:57 点击:532 好评:0

    美国国家人类基因组研究院(NHGRI)3月15日宣布了大规模测序研究网络(Large-ScaleSequencingResearchNetwork)的三个新的优先研究计划。 优先级最高的计划将会分析由国际HapMap计划捐赠的其结构变异性大于已鉴定的SNP的48个人类DNA样本。 第二个计划将会针对...

  • [分子生物学] whole-mount in situ hybridization 日期:2006-03-16 12:45:44 点击:757 好评:0

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  • [分子生物学] DpERK Whole-Mount Immunohistochemistry 日期:2006-03-16 12:42:10 点击:458 好评:0

    This protocol has been optimized for 6 – 10 dpc embryos. Important points: Embryos should be dissected and quickly transferred to fixative as phosphorylation of MAPK is very labile. Fixation must be enough to preserve the diphosphorylatio...

  • [分子生物学] Double in Situ Hybridization 日期:2006-03-16 12:41:50 点击:400 好评:0

    (adapted from Rosen and Beddington [1993] Trends in Genetics 9, 162-167) Double in situ hybridization is performed by hybridizing two different probes (digoxigenin and fluorescein) labeled at the same time. One antibody and chromogenic sub...

  • [分子生物学] Double in Situ Fluorescent Hybridization 日期:2006-03-16 12:41:07 点击:383 好评:0

    Double in situ hybridization is performed by hybridizing two differently labeled probes (digoxigenin and fluorescein labeled) at the same time. After the 1st antibody is used to visualize a probe, it is inactivated and then 2nd antibody an...

  • [分子生物学] DpERK Whole-Mount Immunohistochemistry 日期:2006-03-16 12:39:59 点击:423 好评:0

    This protocol has been optimized for 6 – 10 dpc embryos. Important points: Embryos should be dissected and quickly transferred to fixative as phosphorylation of MAPK is very labile. Fixation must be enough to preserve the diphosphorylatio...

  • [分子生物学] Whole Mount BrdU Staining 日期:2006-03-16 12:38:50 点击:542 好评:0

    Dissect embryos in cold PBS containing 2 mM EGTA. Day 9 embryos and older should have the brain punctured to allow solutions in and out. Fix embryos in 10-15 ml of fresh, cold 4% paraformaldehyde/2 mM EGTA in PBS for two hours at 4°C with...

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