琼脂糖产品（ Agarose Products ） 生产商：美国 Ⅰ . 属分子生物学级别的琼脂糖： SeaKem LE ， SeaPlaque 和 NuSieve 3:1 。 属遗传学技术级别的琼脂糖： SeaKem GTG ， SeaPlaque GTG ， NuSieve GTG ， Seakem Gold 和 Incert 。 Ⅱ . 琼脂糖分类，如下...

ProtocolforDNASequencingviaPCR CleanPCRproduct 1.Add100μlPCRproductstoa1.5mlmicrocentrifugetube.Add1000μlResi nandmixbyvortex3minutes. 2.Takeonedisposablesyringe,removetheplungerandplugonecolumn(forDN Apurification)onthetipofsyringe.Trans...

Zinc is an essential trace element in all forms of life. Despite its critical importance in maintaining such basic functions as proliferation and cell growth, little is known about how zinc is acquired, stored, and utilized by the cell. Re...

美国国家人类基因组研究院（NHGRI）3月15日宣布了大规模测序研究网络（Large-ScaleSequencingResearchNetwork）的三个新的优先研究计划。 优先级最高的计划将会分析由国际HapMap计划捐赠的其结构变异性大于已鉴定的SNP的48个人类DNA样本。 第二个计划将会针对...

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This protocol has been optimized for 6 – 10 dpc embryos. Important points: Embryos should be dissected and quickly transferred to fixative as phosphorylation of MAPK is very labile. Fixation must be enough to preserve the diphosphorylatio...

(adapted from Rosen and Beddington [1993] Trends in Genetics 9, 162-167) Double in situ hybridization is performed by hybridizing two different probes (digoxigenin and fluorescein) labeled at the same time. One antibody and chromogenic sub...

Double in situ hybridization is performed by hybridizing two differently labeled probes (digoxigenin and fluorescein labeled) at the same time. After the 1st antibody is used to visualize a probe, it is inactivated and then 2nd antibody an...

This protocol has been optimized for 6 – 10 dpc embryos. Important points: Embryos should be dissected and quickly transferred to fixative as phosphorylation of MAPK is very labile. Fixation must be enough to preserve the diphosphorylatio...

Dissect embryos in cold PBS containing 2 mM EGTA. Day 9 embryos and older should have the brain punctured to allow solutions in and out. Fix embryos in 10-15 ml of fresh, cold 4% paraformaldehyde/2 mM EGTA in PBS for two hours at 4°C with...