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[Enzymology] Annexin V Staining Protocol 日期：2005-07-18 00:00:00 点击：331 好评：0
Friday, October 17, 2003 Description Annexin V Staining Protocol Procedure 1. Wash cells twice with cold PBS and then resuspend cells in 1X Binding Buffer at a concentration of ~1 x 10 6 cells/ml. 2. Transfer 100 l of the solution (~1 x 10...
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[Enzymology] Radioiodination of cell surface proteins 日期：2005-07-18 00:00:00 点击：167 好评：0
Friday, October 17, 2003 Description Radioiodination of cell surface proteins Procedure 1. Wash cells at least three times with PBS and resuspend in 1 ml of PBS cntg. glucose at 0.5 - 2 x 107 cells/ml) in 15 ml conical tube, hold on ice 2....
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[Enzymology] Radioiodination of protein in solution 日期：2005-07-18 00:00:00 点击：187 好评：0
Friday, October 17, 2003 Description Addition of oxidizing reagents (such as chloramine-T or peroxidase+H202) converts I- to I+ or I3-. This highly reactive molecule attacks o-position of tyrosine (or in some case, histidine is also labele...
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[Enzymology] In vitro Sphingomyelinase Assay 日期：2005-07-18 00:00:00 点击：235 好评：0
Friday, October 17, 2003 Description In vitro Sphingomyelinase Assay Procedure Isolating cellular enzyme 1) Grow 5 x 108 cells under regular growth conditions. 2) Pellet cells and wash one time with ice cold PBS. 3) Resuspend pellet in 10...
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[Enzymology] Conjugation of Antibody to HRP 日期：2005-07-18 00:00:00 点击：170 好评：0
Friday, October 17, 2003 Description Conjugation of Antibody to HRP Procedure A. Preparation of Thiolated Antibody 1. Concentrate the antibody to 5-7 mg/ml 2. Dissolve 10 mg of SATA in 1 ml DMF (SATA:DMF) 3. Add 3.0 ml SATA:DMF per mg of a...
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[Enzymology] Kinase end-labeling of DNA 日期：2005-07-18 00:00:00 点击：168 好评：0
Wednesday, October 08, 2003 Description Typical 5'-kinase labeling reactions included the DNA to be labeled, [[gamma]]-32-P-dATP, T4 polynucleotide kinase, and buffer (1). After incubation at 37degC, reactions are heat inactivated by incub...