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[Immunology] CD3/ CD4 /CD8 T cell subset counts 日期：2005-07-18 00:00:00 点击：290 好评：0
Friday, October 17, 2003 Description Procedure for CD3/ CD4 /CD8 T cell subset counts using CD3-FITC / CD4-PE / CD8-PECy5 antibody Procedure 1.One 5 ml round bottom tube (Falcon) is taken per blood sample to be analyzed. The tube is labele...
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[Immunology] DNA Staining for Cell Cycle Analysis By flow Cytometry 日期：2005-07-18 00:00:00 点击：183 好评：0
Thursday, October 16, 2003 Description This method of DNA staining utilizes ethanol to fix the cells and permeabilize the membrane, which allows the dye (Propidium Iodide) to enter the cells. Propidium Iodide (PI) is a DNA-binding fluoroch...
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[Immunology] Direct Immunofluorescence Labeling 日期：2005-07-18 00:00:00 点击：225 好评：0
Thursday, October 16, 2003 Description This is the method for direct immunofluorescence labeling; that is, the antibodies have the fluorescent dye attached. Direct labeling is simpler and quicker than indirect labeling. Procedure Adjust th...
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[Immunology] IMMUNOCYTOCHEMISTRY 日期：2005-07-18 00:00:00 点击：190 好评：0
Thursday, October 16, 2003 Description Procedures for cell plating, fixing, antibody incubation and mounting Procedure A. Plating: To sterilize glass coverslips, dip in ethanol and flame. We use 22x22x1 mm3 coverslips and put them in 6-wel...