Sporulation Protocol

Tuesday, November 18, 2003

Description
One of the suggested protocol

Procedure
Grow up a small (5mL) overnight culture in YEPD (rich glucose media), 30C.
Count the cells the next day, and dilute the culture down to 1E6 cells/mL, again into a total volume of 5 mL of rich media.
Let the new culture grow at 30 deg C until it reaches the end of log phase, which is 8-9E7 cells/mL.
Spin down the cells and wash them once with sterile water.
Resuspend the cells in sporulation media (2% potassium acetate, pH 7.0) supplemented with necessary nutrients. Set up this culture so that the cells are at a concentration of 5E7 cells/ mL, and a total volume of 2.5 mL.
Let the cultures sporulate 5-7 days at 23 deg C.

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Sporulation Protocol