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Yeast Vivisection

时间:2005-07-18 00:00来源:Scienceboard.net 作者:admin 点击: 183次
Tuesday, November 18, 2003

Description
Yeast Vivisection

Procedure
Spin down 0.5 mL of sporulation culture and resuspend in 1 ml water.
Wash with sterile water.
Resuspend in 50 L of zymolyase solution (0.5 mg/mL in 1M sorbitol).
Incubate for 8-10 minutes at 30C.
Slowly add 0.8 mL sterile water to the tube and place it on ice.
Spread some on a plate and dissect

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