Preparing Serum from Whole Blood

Monday, May 03, 2004

Description
Preparing Serum from Whole Blood

Procedure
Put the blood at 37 for 30-60 min to clot.

3. Pull the rubber stopper off the top, and use a long Pasteur pipette to "ring" the clot, i.e. separate it from the sides of the glass tube. Alternatively, just invert the tube; if the clot pulls free from the glass walls of the tube, then you don't have to ring the clot. Set the tubes at 4 overnight to allow the clot to contract.

4. Decant the serum away from the clot into a new centrifuge tube. Some red blood cells and bits of clot will come along with the serum. Spin the serum 3,000 RPM for 10 min to remove the remaining clots/red blood cells and other insoluble material.

5. Make nicely labelled aliquots and store at -80. The antibody should be stable for many years. A working solution can be stored at 4. If azide won't interfere with your applications, you should add 5-10 mM sodium azide to this working solution tookeep bacteria from growing in it.

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Preparing Serum from Whole Blood