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  • Western Blots 日期:2003-11-20 00:00:00 点击:2196 好评:2

    INTRODUCTION Electrophoresis is the migration of charged molecules in solution in response to an electric field. Their rate of migration depends on the strength of the field; on the nett charge, size and shape of the molecules and also on...

  • Preparation and Staining of Frozen  日期:2004-03-11 14:45:26 点击:720 好评:0

    Preparation and Staining of Frozen Tissue Sections I. Preparation of Frozen Sections for Sectioning Materials neeed: 2-methylbutane (isopentane) Liquid Nitrogen Dry ice Peel-Away?/sup base molds Frozen tissue matrix (OCT?/sup or Cryomatrix...

  • EMSA using ds Oligonucleotides 日期:2003-11-20 00:00:00 点击:840 好评:0

    Solutions 10X Annealing Buffer 200 mM Tris 8.0 200 ml 1M Tris pH 8.0 10 mM EDTA 8.0 20 ml .5M EDTA pH 8.0 500 mM NaCl 100 ml 5M NaCl 280 ml Q store at room temperature 10X Klenow Buffer 500 mM Tris 7.5 500 ml 1M Tris pH 7.5 100 mM MgCl2 10...

  • Basic Immunohistochemistry Protocol 日期:2003-11-20 00:00:00 点击:918 好评:0

    General Note:Protocols for immunohistochemistry vary widely, due to the differences between antigens and their recognition by antibody. Some epitopes are destroyed by the high temperatures and organic solvents used in paraffin fixation, wh...

  • 蒲慕明先生对神经所的2005贺词 日期:2005-01-23 16:19:54 点击:851 好评:8

    Dear members of ION: Happy New Year to all! This is the beginning of the sixth year of ION’s existence, and I am happy to say that we are well on our way to reach our goal of becoming a world-class institution by 2010. A stream of first-r...

  • 蒲慕明--Cultural reflections 日期:2004-07-01 20:48:01 点击:787 好评:0

    Nature 428, 204 - 205 (11 March 2004); doi:10.1038/ 428204a Cultural reflections MU-MING POO Mu-ming Poo is head of the Division of Neurobiology, Department of Molecular and Cell Biology at University of California, Berkeley, California, U...

  • 美国开发“大脑伟哥” 人类记忆力将被改变 日期:2004-03-15 12:56:05 点击:1000 好评:0

    最近,一种被戏称为“大脑伟哥”的能够提高记忆力的药物,即将在美国通过人体试验,预计可能在五年内投入市...

  • 又一造假事件---细胞期刊注销一篇论文 日期:2004-02-24 00:00:00 点击:892 好评:0

    又一造假事件 科研道德:成也“细胞”,败也“细胞” 因数据作假,哥伦比亚大学一研究学者注销其在"细胞"期刊上发表的论文。该项消息一传出即震惊研究生命科学领域的学者。 Gary Struhl博士在美国哥伦比亚大学所率领的研究小组以研究细胞信息传递路径的整合驰...

  • Grisha Perelman needs no awards, &n 日期:2004-01-29 00:00:00 点击:887 好评:0

    Grisha Perelman needs no awards, no funds and..... no jobs just math but he has not gone insane (yet) Nature 427, 388 - 389 (29 January 2004); doi:10.1038/427388a Mathematics: The reluctant celebrity A reclusive Russian claims to have solv...

  • 蒲慕明教授对神经所2004的新年祝词 日期:2004-01-10 00:00:00 点击:1089 好评:-2

    Dear Members of ION: At the beginning of 2004, I wish everyone a happy and fruitful New Year. On my way back to Berkeley, I attended the Advisory Board meeting of the Institute of Molecular Biology of Academia Sinica in Taiwan. I found out...

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