Curriculum vitae J. Klumperman

Family name:   Klumperman

First name:   Judith

Sex:    female

Work address:   Cell Microscopy Centre
    Department of Cell Biology,
    University Medical Center Utrecht (UMCU),
    AZU G02.525, Heidelberglaan 100
    3584CX Utrecht, The Netherlands
    Phone: 31-30-2506550/6551
    Fax: 31-30-2541797
    Email: J.Klumperman@lab.azu.nl

Date of birth:   August 9, 1961

Place of birth:   Delft, The Netherlands

Nationality:   Dutch

Start university training:  September 1979, University of Leiden

Discipline:   Biology

Graduate:   April 1985

PhD:    May 1990

Employment record
April 1985-September 1989: PhD at the Laboratory for Electron Microscopy, Medical Faculty, University of Leiden (UL), The Netherlands (Promotor: Prof. W. T. Daems) and the Department for Biochemistry, Academic Medical Centre, University of Amsterdam, The Netherlands (Promotor: Prof. J.M. Tager).
PhD thesis: Transport of lysosomal and brush border enzymes in polarized colon carcinoma cell lines.
September 1989-October 1992: Post-doctoral fellowship at the Department of Cell Biology, Center for Electron Microscopy, Medical Faculty, University of Utrecht, the Netherlands. (Supervisor: Prof. H.J. Geuze) and Prof. K. Von Figura, Department of Biochemistry, Georg-August University, Göttingen, Germany.
October 1992-July 1997: Assistant professor (tenure) at the Department of Molecular and Cellular Neurobiology, Faculty Biology, Free University (VU), Amsterdam, The Netherlands.
July 1997: Assistant professor (tenure) at the Department of Cell Biology, University Medical Centre Utrecht (UMCU), The Netherlands.
July 1999-July 2001: Associate professor (tenure) at the Department of Cell Biology, UMCU, The Netherlands.
July 2001-present: Full professor Cell Biology and Head of the Cell Microscopy Centre, Department of Cell Biology, UMCU, The Netherlands.

Professional Societies
Dutch Society for Microscopy
Dutch Society for Cell Biology
American Society for Cell Biology

Research interest
My general research interest is to understand the mechanisms of vesicular trafficking in relation to organelle formation and functioning in healthy and diseased cells. My current research focuses on lysosome biogenesis. Lysosomes have long been considered as the terminal degradative compartments of the cell, but this view is now completely changed by an overwhelming amount of data demonstrating multiple specialized functions of lysosomes. Resolving the pathways of lysosome assembly is therefore eminent to understand the molecular basis of the growing group of genetic disorders that are converted to lysosomal dysfunctions.
My research has added to the functional characterization of transport-machinery proteins, e.g. Rab , coat- and SNARE proteins, in physiological and pathological conditions. Two important milestones in my work are 1. the ‘concentration-by-exclusion’ model for transport of secretory proteins from the ER to the Golgi complex and 2. establishing the presence of Golgi-enzymes in Golgi-associated COPI vesicles. Both findings have important implications for understanding the mode of transport through this complex part of the secretory pathway. In addition, my work contributed to the identification of the mannose 6-phosphate receptor-pathway for lysosomal enzyme delivery and the discovery of a novel, AP-3 mediated pathway that transports lysosomal membrane proteins from tubular sorting endosomes to lysosomes.
Highlights in my work on the endocytic pathway are the identification of a Hrs/clathrin-coat on endosomes (the precursors of lysosomes) that is necessary for the concentration and sorting of growth factor receptors to the lysosomal degradation pathway. We are currently extending these studies to specific types of breast cancer, in which receptor degradation is impaired resulting in continuous signaling for growth.
During the course of my career I have specialized in the application of microscopy methods, especially immuno-electron microscopy, to address biomedical questions in the membrane traffic field. From 2001 on I am Director of the internationally renowned Cell Microscopy Center for the application of advanced microscopy studies in cutting edge bio-medical research. During my studies I have experienced the limitations of a static approach to study dynamic events. Recently, I introduced a correlative microscopy method that allows a direct combination of live-cell imaging with the most sensitive immunoEM method to date.

Management
1989-1990: Board member of the Department of Cell Biology, Medical Faculty, University of Utrecht.
1995-1997: Board member of the Department of Molecular and Cellular Neurobiology, Faculty Biology, Free University Amsterdam.
1998-present: Member of the daily board/management team of the Department of Cell Biology, UMCU.
2000: Interim-project leader at the Graduate School Institute of Biomembranes: Membrane specificity and organelle biogenesis.
2001: Project leader at the Graduate School Institute of Biomembranes: Membrane specificity and organelle biogenesis.
2001: Chair of the Cell Microscopy Centre, UMC Utrecht
2001: Board member of the Dutch Society for Microscopy
2002-2006: Member of the Council for Medical Sciences (Raad voor Medische Wetenschappen) of the Royal Dutch Academy of Arts and Sciences (KNAW).
2002-2004: member of the committee: Financing biomedical research. Royal Dutch Academy of Arts and Sciences (KNAW).
June 2002-present: Vice-president of the Dutch Society for Microscopy
2004: Member of the committee ‘Internationalisering’. Strategic impulse, UMC Utrecht.
2004: Board member Graduate School Institute of Biomembranes:
2004: Member of the Talma Eykman prize jury.
2004: Chair of the board of the Graduate School Institute of Biomembranes

Organization International Conferences
1999: Co-organiser of the international mini-symposium: ‘Cell Biology of Disease’. AZU-UMCU, Utrecht, The Netherlands.
2000: Co-organizer and chair of the mini-symposium ‘Membrane Traffic’ of the European Electron Microscopy congress EUREM 2000, Brno, Czech Republic, July 9-14, 2000.
2001: Co-organizer of and chair at the international symposium Molecular mechanisms in membrane transport. June 28-29, Utrecht, The Netherlands.
2001: Vice-president of the organizing committee of the annual meeting of the Dutch Society for Microscopy (NVvM). December 13-14, Papendal Conference Center, The Netherlands
2002: Member of the organizing committee of the 44th International Symposium of the Society for Histochemistry ‘Proteomics in situ: imaging proteins at work’. Vlissingen, 25-28 September 2002, The Netherlands.
2002: Co-organizer and chair of the mini-symposium ‘Organelle Biogenesis and Inheritance’ at the 42nd Annual Meeting of the American Society for Cell Biology (ASCB). 14-18 December 2002, San Francisco, USA.
2004: Chair mini-symposium: Transport through the Golgi apparatus. Keystone symposium: Golgi apparatus and secretory pathway of eukaryotic cells. January 20 – january 25, Beaver Run Resort, Breckenridge, Colorado. Organizers: L.A. Staehelin, B. Glick & K.E. Howell
2004: Member of the organizing committee of the Royal Dutch Academy of Arts and Sciences (KNAW) symposium: Financing biomedical research in the Netherlands (De financiering van (bio) medisch wetenschappelijk onderzoek in Nederland). June 17. Amsterdam, The Netherlands.
2004: Member of the Scientific Advisory Board of the 2004 Electron Microscopical Society (EMC) Congress. August 22-27. Antwerpen, Belgium
2004: Member of the Programme committee of the 2004 European Life Science Organization (ELSO) annual meeting. September 4-8. Nice, France.

Other academic activities
- Reviewer for: Journal of Cell Biology, EMBO Journal, Nature Cell Biology, Molecular Biology of the Cell, Journal of Cell Science, Traffic, Histochemistry & Cell Biology.
- Grant reviewer for: the Dutch Society for Scientific research (NWO), Wellcome Trust (UK), Medical Research Council (UK), Telethon (Italy), KNAW.
1996-1997: Co-organiser of the weekly seminar series at the Department of Molecular and Cellular Neurobiology, Faculty Biology, Free University, Amsterdam.
1997: Member of the organising committee of the AIO/OIO-meeting of the Research Institute Neurosciences Amsterdam.
June 1999: J. Klumperman. Arts and artifacts of electron microscopy. Trends in Cell Biology, 1999, Vol. 9:247. Review on ‘Biomedical Electron Microscopy, Illustrated methods and Interpretations, by A.B. Maunsbach and B.A. Afzelius, ISBN0124806104. (on invitation)
February 2000: Cover design of Molecular Biology of the Cell, the international journal of the American Society for Cell Biology (ASCB), Vol. 11, No. 2. (on invitation)
July 2000: J. Klumperman. Immuno-electronenmicroscopie, Analyse, vakblad Nederlandse Vereniging van bio-Medische Laboratoriummedewerkers. November 2000, 270-274. (on invitation).
2001: Corresponding jury member of the committee ‘Stichting Electronenmikroskopie Nederland (SEN)’ (= Dutch society for Electron microscopy) awarding the SEN-prize 2001.

Editorships
2002-present: Traffic
2002-present: Histochemistry and Cell Biology
2003-present: Biology of the Cell
2004: Section editor of the Membranes and organelles (August 2004) issue of Current Opinion in Cell Biology