总之,我国基因芯片研究也紧跟国际前沿,力争在此高新技术领域里有一席之地,它将对我国生命科学研究,医学诊断,新药筛选具有革命性的推动作用,也将对我国人口素质、农业发展、环境保护等作出具大的贡献,同时带动我国科学整体进步,为各相关高科技产业创造机会。基因芯片将成为21世纪最令人注目的高新技术领域之一,将使人类早日进入生物信息时代。 [参考文献] 1. 徐炳森、邵健忠 几种新型生物芯片的研究进展。生物化学与生物物理进展。2000.27(3):251-253. 2. Pease AN, Solas D, sullivan EJ,et al. Light-generated oligonucleotide arrays for rapid DNA sequecences analysis. Proc Natl Acad sci USA, 1994, 91(11):5022-5026 3. Schena M, Heller KA, Theriault TP, et al. Microarrays: biotechnology's discovery platform for functional genomics. TIB Tech, 1998, 16(3):301-306 4. Marshall A, Hodgson J. DNA chips: An array of possibilities. Nat Biotechnol, 1998, 16(1):27-31. 5. Shohe pinov MS. Nucleic Acids Res.1997, 25:1155-1161 6. Cheng J, Sheldom EL, Wu L. Preparation and hybridization analysis of DNA/RNA from E, coli on microfabricated bioelectronic chips. Nat Biotechnol, 1998, 16(6):541-546 7. Chee M, Yang R, Hubbell E,et al.. Accessing genetic information with high-density DNA arrays.Science, 1996, 274:610-613 8. Hacia JG, Makalowski W, Edgemon k, et al. Evolutionary sequence comparisons using high-density oligonucleotide arrays. Nature Genetics, 1998, 18(3):155-158. 9. Schena M, Shalon D, Davis RW, et al. Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science, 1995, 270(5235):467-470. 10. Schena M shalon D, Heller R, et al. Parallel human genome analysis: microarray-based expression monitoring of 1000 genes. Pro Natl Acad sci USA, 1996, 93(20):457-460. 11. Goffeau A, Molecular fish on chips. Nature, 1997, 385(6613):202-203 12. Heller RA,Schena M,Chai A et al.Discovery and analysis of inflammatory disease-related genes using cDNA microarrays.Proc Natl Acad Sci USA,1997,94:2150-2155 13. Jells CL.Defining critical residues in the epitope for HIV-neutralizing monoclonal antibody using phage display and peptide array technologies [J].Gene ,1993,137910:63-68 14. Lipshutz D,Morris D,Chee M et al.Using oligonucleotide probe array to access genetic diversity. Biotechniques,1995,19(3):422-447 15. Editorial. To affinity……and beyond! Nat Genet, 1996, 14(4):367. 16. Andrew M. Nat Biotechnal, 1998,16 17. Editorial: Getting hip to the chip. Nature Genetics, 1998, 18(3):195-197. (责任编辑:泉水) |