基因序列研究的通用过程

Program Function Blastn Nucleotide query against nucleotide database Blastp Protein query against protein database Blastx Nucleotide query against protein databas" />

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基因序列研究的通用过程

时间:2005-08-02 10:45来源:本站原创作者:runma 点击: 1489次

The Flowchart of DNA sequence analysis

Program	Function
Blastn	Nucleotide query against nucleotide database
Blastp	Protein query against protein database
Blastx	Nucleotide query against protein database
Pairwise blast	Comparison of two nucleotide or protein seqs
CD search	Conserved domain search

Domain search:

l Most genes/proteins in the database do not have an assigned function. Such sequences show “no hits” or have similarity to conserved hypothetical proteins in the BLAST search results.

l One way to assign a tentative function to an unknown protein is to search for conserved domains in that protein because domains are usually the functional units in proteins.

l Strategy: similar to BLAST, local alignment analysis. Hidden Markov Model is used.

Signal peptide prediction

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