80. Kreimer DI, Szosenfogel R, Goldfarb D, Silman I, Weiner L. (1994) Two-state transition between molten globule and unfolded states of acetylcholinesterase as monitored by electron paramagnetic resonance spectroscopy. Proc Natl Acad Sci U S A. 91(25):12145-9. 81. Kreimer DI, Dolginova EA, Raves M, Sussman JL, Silman I, Weiner L. (1994) A metastable state of Torpedo californica acetylcholinesterase generated by modification with organomercurials. Biochemistry. 33(48):14407-18. 82. Eichler J, Kreimer DI, Varon L, Silman I, Weiner L. (1994) A "molten globule" of Torpedo acetylcholinesterase undergoes thiol-disulfide exchange. J Biol Chem. 269(48):30093-6. 83. Silman I, Harel M, Axelsen P, Raves M, Sussman JL. (1994) Three-dimensional structures of acetylcholinesterase and of its complexes with anticholinesterase agents. Biochem Soc Trans. 22(3):745-9. Review. 84. Gilson MK, Straatsma TP, McCammon JA, Ripoll DR, Faerman CH, Axelsen PH, Silman I, Sussman JL. (1994) Open "back door" in a molecular dynamics simulation of acetylcholinesterase. Science. 263(5151):1276-8. 85. Weiner L, Kreimer D, Roth E, Silman I. (1994) Oxidative stress transforms acetylcholinesterase to a molten-globule-like state. Biochem Biophys Res Commun. 198(3):915-22. 86. Eichler J, Anselment A, Sussman JL, Massoulie J, Silman I. (1994) Differential effects of "peripheral" site ligands on Torpedo and chicken acetylcholinesterase. Mol Pharmacol. 45(2):335-40. 87. Mehlert A, Varon L, Silman I, Homans SW, Ferguson MA. (1994) Novel GPI structures of the membrane anchor of acetylcholinesterase from the electric organ of Torpedo californica. Braz J Med Biol Res. 27(2):243-8. 88. Axelsen PH, Harel M, Silman I, Sussman JL. (1994) Structure and dynamics of the active site gorge of acetylcholinesterase: synergistic use of molecular dynamics simulation and X-ray crystallography. Protein Sci. 3(2):188-97. 89. Mehlert A, Varon L, Silman I, Homans SW, Ferguson MA. (1993) Structure of the glycosyl-phosphatidylinositol membrane anchor of acetylcholinesterase from the electric organ of the electric-fish, Torpedo californica. Biochem J. 296 ( Pt 2):473-9. 90. Harel M, Schalk I, Ehret-Sabatier L, Bouet F, Goeldner M, Hirth C, Axelsen PH, Silman I, Sussman JL. (1993) Quaternary ligand binding to aromatic residues in the active-site gorge of acetylcholinesterase. Proc Natl Acad Sci U S A. 90(19):9031-5. 91. Ripoll DR, Faerman CH, Axelsen PH, Silman I, Sussman JL. (1993) An electrostatic mechanism for substrate guidance down the aromatic gorge of acetylcholinesterase. Proc Natl Acad Sci U S A. 90(11):5128-32. 92. Sussman JL, Harel M, Silman I. (1993) Three-dimensional structure of acetylcholinesterase and of its complexes with anticholinesterase drugs. Chem Biol Interact. 87(1-3):187-97. 93. Cygler M, Schrag JD, Sussman JL, Harel M, Silman I, Gentry MK, Doctor BP. (1993) Relationship between sequence conservation and three-dimensional structure in a large family of esterases, lipases, and related proteins. Protein Sci. 2(3):366-82. Review. 94. Mehlert A, Silman I, Homans SW, Ferguson MA. (1993) The structure of the glycosylphosphatidylinositol anchor from Torpedo californica acetylcholinesterase. Biochem Soc Trans. 21(1):43S. 95. Massoulie J, Sussman J, Bon S, Silman I. (1993) Structure and functions of acetylcholinesterase and butyrylcholinesterase. Prog Brain Res. 98:139-46. Review. 96. Dolginova EA, Roth E, Silman I, Weiner LM. (1992) Chemical modification of Torpedo acetylcholinesterase by disulfides: appearance of a "molten globule" state. Biochemistry.31(48):12248-54. 97. Harel M, Sussman JL, Krejci E, Bon S, Chanal P, Massoulie J, Silman I. (1992) Conversion of acetylcholinesterase to butyrylcholinesterase: modeling and mutagenesis. Proc Natl Acad Sci U S A. 89(22):10827-31. 98. Eichler J, Silman I, Anglister L. (1992) G2-acetylcholinesterase is presynaptically localized in Torpedo electric organ. J Neurocytol. 21(10):707-16. 99. Duval N, Bon S, Silman I, Sussman J, Massoulie J. (1992) Site-directed mutagenesis of active-site-related residues in Torpedo acetylcholinesterase. Presence of a glutamic acid in the catalytic triad. FEBS Lett. 309(3):421-3. 100.Ollis DL, Cheah E, Cygler M, Dijkstra B, Frolow F, Franken SM, Harel M, Remington SJ, Silman I, Schrag J, et al. (1992) The alpha/beta hydrolase fold. Protein Eng. 5(3):197-211. 101.Eichler J, Toker L, Silman I. (1991) Effect of heat shock on acetylcholinesterase activity in chick muscle cultures. FEBS Lett. 293(1-2):16-20. 102.Harel M, Su CT, Frolow F, Ashani Y, Silman I, Sussman JL. (1991) Refined crystal structures of "aged" and "non-aged" organophosphoryl conjugates of gamma-chymotrypsin. J Mol Biol. 5;221(3):909-18. 103.Sussman JL, Harel M, Frolow F, Oefner C, Goldman A, Toker L, Silman I. (1991) Atomic structure of acetylcholinesterase from Torpedo californica: a prototypic acetylcholine-binding protein. Science. 253(5022):872-9. 104.Harel M, Su CT, Frolow F, Silman I, Sussman JL. (1991) Gamma-chymotrypsin is a complex of alpha-chymotrypsin with its own autolysis products. Biochemistry. 30(21):5217-25. 105.Espinoza B, Silman I, Arnon R, Tarrab-Hazdai R. (1991) Phosphatidylinositol-specific phospholipase C induces biosynthesis of acetylcholinesterase via diacylglycerol in Schistosoma mansoni. Eur J Biochem. 195(3):863-70. 106.Steinberg N, Roth E, Silman I. (1990) Torpedo acetylcholinesterase is inactivated by thiol reagents. Biochem Int. 21(6):1043-50. 107.Eichler J, Silman I, Gentry MK, Anglister L. (1990) Immunocytochemical localization of phosphatidylinositol-anchored acetylcholinesterase in excitable membranes of Torpedo ocellata. Brain Res Mol Brain Res. 8(3):213-8. 108.Grunwald J, Segall Y, Shirin E, Waysbort D, Steinberg N, Silman I, Ashani Y. (1989) Aged and non-aged pyrenebutyl-containing organophosphoryl conjugates of chymotrypsin. Preparation and comparison by 31P-NMR spectroscopy. Biochem Pharmacol. 38(19):3157-68. 109.Steinberg N, van der Drift AC, Grunwald J, Segall Y, Shirin E, Haas E, Ashani Y, Silman I. (1989) Conformational differences between aged and nonaged pyrenebutyl-containing organophosphoryl conjugates of chymotrypsin as detected by optical spectroscopy. Biochemistry. 28(3):1248-53. 110.Steinberg N, Grunwald J, Roth E, August R, Haas E, Ashani Y, Silman I. (1989) Conformational differences between aged and non-aged organophosphoryl conjugates of chymotrypsin. Prog Clin Biol Res. 289:293-304. 111.Sussman JL, Harel M, Frolow F, Varon L, Toker L, Futerman AH, Silman I. (1988) Purification and crystallization of a dimeric form of acetylcholinesterase from Torpedo californica subsequent to solubilization with phosphatidylinositol-specific phospholipase C. J Mol Biol. 203(3):821-3. 112.Eghbali M, Silman I, Robinson TF, Seifter S. (1988) Visualization of collagenase-sensitive acetylcholinesterase in isolated cardiomyocytes and in heart tissue. Cell Tissue Res. 253(2):281-6. 113.Inestrosa NC, Fuentes ME, Anglister L, Futerman AH, Silman I. (1988) A membrane-associated dimer of acetylcholinesterase from Xenopus skeletal muscle is solubilized by phosphatidylinositol-specific phospholipase C. Neurosci Lett. 90(1-2):186-90. 114.Espinoza B, Tarrab-Hazdai R, Silman I, Arnon R. (1988) Acetylcholinesterase in Schistosoma mansoni is anchored to the membrane via covalently attached phosphatidylinositol. Mol Biochem Parasitol. 29(2-3):171-9. 115.Silman I, Futerman AH. (1987) Modes of attachment of acetylcholinesterase to the surface membrane. Eur J Biochem. 170(1-2):11-22. Review. 116.Doctor BP, Toker L, Roth E, Silman I. (1987) Microtiter assay for acetylcholinesterase. Anal Biochem. 166(2):399-403. 117.Futerman AH, Raviv D, Michaelson DM, Silman I. (1987) Differential susceptibility to phosphatidylinositol-specific phospholipase C of acetylcholinesterase in excitable tissues of embryonic and adult Torpedo ocellata. Brain Res. 1987 Jul;388(2):105-12. 118.Low MG, Futerman AH, Ackermann KE, Sherman WR, Silman I. (1987) Removal of covalently bound inositol from Torpedo acetylcholinesterase and mammalian alkaline phosphatases by deamination with nitrous acid. Evidence for a common membrane-anchoring structure. Biochem J. 241(2):615-9. 119.Silman I, Futerman AH. (1987) Posttranslational modification as a means of anchoring acetylcholinesterase to the cell surface. Biopolymers. Suppl:S241-53. 120.Goldlust A, Arnon R, Silman I, Tarrab-Hazdai R. (1986) Acetylcholinesterase of Schistosoma mansoni: purification and characterization. J Neurosci Res. 15(4):569-81. 121.Futerman AH, Low MG, Michaelson DM, Silman I. (1985) Solubilization of membrane-bound acetylcholinesterase by a phosphatidylinositol-specific phospholipase C. J Neurochem. 45(5):1487-94. 122.Barton PL, Futerman AH, Silman I. (1985) Arrhenius plots of acetylcholinesterase activity in mammalian erythrocytes and in Torpedo electric organ. Effect of solubilization by proteinases and by a phosphatidylinositol-specific phospholipase C. Biochem J. 231(1):237-40. 123.Futerman AH, Low MG, Ackermann KE, Sherman WR, Silman I. (1985) Identification of covalently bound inositol in the hydrophobic membrane-anchoring domain of Torpedo acetylcholinesterase. Biochem Biophys Res Commun. 129(1):312-7. 124.Page JD, Wilson IB, Silman I. (1985) Butyrylcholinesterase: inhibition by arsenite, fluoride, and other ligands, cooperativity in binding. Mol Pharmacol. 27(4):437-43. (责任编辑:泉水) |