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EM Immunogold Labeling Protocol - P(5)

时间:2006-01-22 15:57来源:ihcworld.com 作者:bioguider 阅读:

 

5% Uranyl Acetate Solution:

To prepare 50 ml,

Uranyl acetate --------------------- 2.5 g

Distilled water --------------------- 50 ml

Cover with foil and stir overnight.

Add 10 drops of glacial acetic acid and Store solution in 4 C for 3 month.

 

Reynold’s Lead Citrate Solution:

To prepare 50 ml, add chemicals in distilled water in following order

Lead nitrate ------------------------------- 1.33 g

Sodium citrate, dihydrate -------------- 1.76 g (solution becomes cloudy when sodium citrate is added)

1N NaOH ----------------------------------- 5 ml  (solution becomes clear when NaOH is added)

Distilled water ----------------------------- 30 ml

Stir for 10 minutes to dissolve and add additional 15 ml of distilled water. Store solution for 3-6 months at 4 C. Note: the mount of NaOH is very important. Too much will make solution cloudy.

 

Epon 812:

 

(责任编辑:泉水)

Final Volume

EM bed-812

DDSA

NMA

DMP-30 (2%)

20.91 ml

10 ml

4.5 ml

6 ml

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