Methods in Neuroscience Research
Foreword by A. Claudio Cuello
With 66 Illustrations
This book is dedicated to the memory of Giovanni Godina,
Emeritus Professor of Veterinary Anatomy at the University of Torino, Italy.
CONTENTS
Foreword . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii
1 Analyses of Intracellular Signal Transduction Pathways in CNS
Progenitor Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Elena Cattaneo and Luciano Conti
2 Confocal and Electron Microscopic Tracking of Internalized
Neuropeptides and/or Their Receptors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Alain Beaudet, Alexander C. Jackson, and Franck Vandenbulcke
3 Transfection Methods for Neurons in Primary Culture . . . . . . . . . . . . . . . . . . 29
Christoph Kaether, Martin Köhrmann, Carlos G. Dotti,
and Francesca Ruberti
4 Polyethylenimine: a Versatile Cationic Polymer for Plasmid-Based Gene
Delivery in the CNS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Barbara A. Demeneix, Gregory F. Lemkine, and Hajer Guissouma
5 Transfection of GABAA Receptor with GFP-Tagged Subunits
in Neurons and HEK 293 Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Stefano Vicini, Jin Hong Li, Wei Jian Zhu