生物行移动版

The Flowchart of DNA sequence analysis

Domain search:

l         Most genes/proteins in the database do not have an assigned function. Such sequences show “no hits” or have similarity to conserved hypothetical proteins in the BLAST search results.

l         One way to assign a tentative function to an unknown protein is to search for conserved domains in that protein because domains are usually the functional units in proteins.

l         Strategy: similar to BLAST, local alignment analysis. Hidden Markov Model is used.

Signal peptide prediction