-
[RNA] Methods for Plant RNA Isolation 日期：2005-07-18 00:00:00 点击：417 好评：0
Friday, November 07, 2003 Description Methods for Plant RNA Isolation Procedure AMES/Chloroform extraction in our lab to extract total RNA from potato leaves for viroid analysis. The protocol is as follows: AMES Buffer (for 200mL):11.7g Na...
-
[RNA] In Vitro Transcription Assay (Run-off&nb 日期：2005-07-18 00:00:00 点击：575 好评：0
Description With this protocol, transcripts that were initiated from specific genes by RNA polymerases prior to permeabilization can be measured. Instead of a nuclear extract, permeabilized cells are used. Procedure A. Permeabilization of...
-
[RNA] RNase Protection and Immunoprecipitation of Nuclear Extracts 日期：2005-07-18 00:00:00 点击：259 好评：0
Tuesday, October 21, 2003 Description RNase Protection and Immunoprecipitation of Nuclear Extracts Procedure A. Prepare Beads (for 15 reactions) 1. Resuspend 60 mg of Protein A-Sepharose CL-4B or 375 l Protein G-Sepharose in 7.5 ml of NET2...
-
[RNA] Complete RNase T1 Digest 日期：2005-07-18 00:00:00 点击：337 好评：0
Tuesday, October 21, 2003 Description RNase T1 is an endonuclease that specifically cleaves 3' of G residues in RNA. This protocol uses RNase T1 for RNA mapping studies. Procedure 1. Dry the RNA sample in either a vacuum centrifuge or a ly...
-
[RNA] RNase Protection Assay 日期：2005-07-18 00:00:00 点击：294 好评：0
Description This protocol can be used to quantitate an mRNA of interest if the RNA probe is present in molar excess over the target mRNA. A labeled RNA transcript is hybridized to an mRNA population and any non-hybridized (single stranded)...
-
[RNA] RNA Primer Extension 日期：2005-07-18 00:00:00 点击：294 好评：0
Monday, October 20, 2003 Description RNA Primer Extension Procedure 1. Precipitate the RNA with 5' end-labeled primer in 0.3 M Sodium Acetate with 2.5 volumes of 100% Ethanol (See Hint #1). 2. Mix well by inversion, microcentrifuge to pell...
-
[RNA] Reverse Transcriptase Assay Using DEAE-Cellulose 日期：2005-07-18 00:00:00 点击：253 好评：0
Monday, October 20, 2003 Description Reverse Transcriptase Assay Using DEAE-Cellulose Procedure 1. Take 10 l of medium from the wells of a 96-well microtiter plate containing a confluent cell culture of the desired cell line and put it int...
-
[RNA] Deprotection of Synthetic RNA 日期：2005-07-18 00:00:00 点击：250 好评：0
Monday, October 20, 2003 Description This protocol describes the desilylation or 2' deprotection of synthetic RNA. Procedure 1. After synthesis on the 1 M scale, take the column apart with pliers and deposit the resin in a glass deprotecti...
-
[RNA] RNase Protection and Immunoprecipitation of Nuclear Extracts 日期：2005-07-18 00:00:00 点击：182 好评：0
Monday, October 20, 2003 Description RNase Protection and Immunoprecipitation of Nuclear Extracts Procedure A. Prepare Beads (for 15 reactions) 1. Resuspend 60 mg of Protein A-Sepharose CL-4B or 375 l Protein G-Sepharose in 7.5 ml of NET2...
-
[RNA] RNase Protection and Immunoprecipitation of Nuclear Extracts 日期：2005-07-18 00:00:00 点击：146 好评：0
Sunday, October 19, 2003 Description RNase Protection and Immunoprecipitation of Nuclear Extracts Procedure A. Prepare Beads (for 15 reactions) 1. Resuspend 60 mg of Protein A-Sepharose CL-4B or 375 l Protein G-Sepharose in 7.5 ml of NET2...