Figure 3. Example of a whole-cell recording from the terminal bulb of the pharynx. A depolarizing step (as shown in the stimulus protocol) elicits an inward current whole cell current. This recording was made from a wild type (N2) pharynx in the presence of 50nM 5-HT.
5. Acknowledgements
Irina Vinogradova contributed to the development of the patch clamp protocols. Enriq Claverol and Christopher Franks developed the automated system for simultaneous acquisition of electrical and video signals. We gratefully acknowledge the pioneering work of the Avery lab who led the way in this field. The Holden-Dye group also acknowledge the support of the Biotechnology and Biological Science Research Council, UK for funding the electrophysiological analysis of C. elegans.
6. References
Avery, L., Raizen, D., and Lockery, S. (1995). Electrophysiological methods. Methods Cell Biol. 48, 251–269. Abstract
Franks, C.J., Pemberton, D., Vinogradova, I., Cook, A., Walker, R.J., and Holden-Dye, L. (2002). The ionic basis of the resting membrane potential and action potential in the pharyngeal muscle of Caenorhabditis elegans. J. Neurophysiol. 87, 954–961. Abstract
Lee, R.N., Lobel, L., Hengartner, M., Horvitz, H.R., and Avery, Y.L. (1997). Mutations in the alpha 1 subunit of an L-type voltage-activated Ca2+ channel cause myotonia in Caenorhabditis elegans. EMBO J. 16, 6066–6076. Abstract Article
Purves, R.D. (1981). Microelectrode Methods for Intracellular Recording and Ionophoresis (Academic Press).
Raizen, D.M., and Avery, L. (1994). Electrical-activity and behavior in the pharynx of Caenorhabditis elegans. Neuron 12, 483–495. Abstract Article
Raizen, D.M., Lee, R.N., and Avery, L. (1995). Interacting genes required for pharyngeal excitation by motor-neuron MC in Caenorhabditis elegans. Genetics 141, 1365–1382. Abstract
Richmond, J., and Jorgensen, E.M. (1999). One GABA and two acetylcholine receptors function at the C. elegans neuromuscular junction. Nat. Neurosci. 2, 791–797. Abstract Article
Shtonda, B., and Avery, L. (2005). CCA-1, EGL-19 and EXP-2 currents shape action potentials in the Caenorhabditis elegans pharynx. J. Exp. Biol. 208, 2177–2190. Abstract Article
Vinogradova, I., Cook, A., and Holden-Dye, L. (2006). The ionic dependence of voltage-activated inward currents in the pharyngeal muscle of Caenorhabditis elegans. Invert Neurosci. Abstract Article
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*Edited by William J. Schafer. WormMethods editor, Victor Ambros. Last revised August 10, 2005. Published May 17, 2006. This chapter should be cited as: Cook, A., et al. Electrophysiological recordings from the pharynx (May 17, 2006), WormBook, ed. The C. elegans Research Community, WormBook, doi/10.1895/wormbook.1.110.1, http://www.wormbook.org.
Copyright: © 2006 Alan Cook, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
§To whom correspondence should be addressed. E-mail: lmhd@soton.ac.uk