Saturday, May 15, 2004

Description
HOXB4 MspI Polymorphism

Procedure
MspI in HOXB4; Clone pH 2-1-3 Bg 2

Template: Genomic DNA 100-200 ng/reaction volume of 25 l

Primer 1. BG2FIN2 '5-AGG ATG TGT GAG AGG CTG G 100 ng/reaction
Primer 2. BG2RINT '5-GCA CAA AAG GAG GAA GGG TC 100 ng/reaction

dNTPs: Final Concentration of 200 M each

Buffer: Final Concentration 2.25 mM MgCl2, 50 mM KCl, 10 mM Tris HCI pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction volume of 25 l
(Perkin-Elmer Amplitaq DNA polymerase 5U/l, Cat No. N808-0145)

PCR Cycling Profile: 94oC (5')
94oC (30"), 60oC (30"), 72oC (1.0') 30 cycles

Product Size:
MspI
560 bp --------->350 bp + 210 bp (+/+ individual)

Ogura, T., C.M. Castiglione, A.J. Pakstis, and K.K. Kidd, 1991. An MspI polymorphism for the HOX2F gene. Nucleic Acids Research 19:1716.


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