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Tuesday, November 18, 2003
Description Yeast Rapid Protein Prep Procedure Sample Buffer: 0.06M Tris-HCl, pH 6.8 10% (v/v) glycerol 2% (w/v) SDS 5% (v/v) 2-mercaptoethanol 0.0025% (w/v) bromophenol blue Grow cells overnight (~1E7 cells/ml) and collect 1.5 ml cells in 1.5 ml microfuge tube (1 minute, 14000xg). Wash cells 1X with water and collect again by centrifugation. Resuspend cells in 100 l sample buffer. Heat at 95 deg C for 5 minutes. Centrifuge 14000xg for 5 minutes. Load ~25 l per lane on an SDS polyacrylamide gel. Recipes Supplies Tips (责任编辑：泉水) |
Yeast Rapid Protein Prep
时间:2005-07-18 00:00来源:Scienceboard.net 作者:admin
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