Routine TEM Staining Protocol for Tissues Fixation: 1. Fix 1 mm tissue blocks in 4% formaldehyde and 1% glutaraldehyde in 0.1 M PB (pH 7.4) for at least 2 hours to overnight. 2. Immerse in 8% (0.2M) sucrose in 0.1 M PB 3x15min or overnight...

Routine TEM Staining Protocol for Cells Fixation: 1. Fix cell suspension or free cells in 4% formaldehyde and 1% glutaraldehyde in 0.1 M PB (pH 7.4) by mixing equal volume of fixative and cell suspension. 2. Transfer cells to centrifuge tu...

铁蛋白免疫电镜技术 （一） 原理 免疫铁蛋白技术是以铁蛋白标记抗体，再以铁蛋白抗体与待检抗原作用。通过电镜检查，观察到铁蛋白抗体所在的位置，即抗原所在。 （二）材料与试剂 1．马脾铁蛋白 2．硫酸铵 3．硫酸镉 4．双异氰酸镉二甲苯(Metaxylene dlisocy...

扫描电镜,透射电镜技术 ㈠ 序言 1．电镜发展史 2．电镜在生物医学中的作用 3．电镜进展：超高压电镜、扫描透射电镜、扫描隧道显微镜、分析电镜等 4．电镜与其它显微镜的区别 ㈡ 透射电镜 1．概述 2. 基本原理 3．结构和操作 ㈢ 扫描电镜 1．概述 2．成像原理...