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[RNA] Native Gel Electrophoresis of RNA-Protein Complexes 日期：2005-07-18 00:00:00 点击：317 好评：0
Sunday, October 19, 2003 Description This protocol is optimized to resolve complexes formed on short (50 to 70 nucleotide) RNA oligonucleotides. Incubation conditions, percentage of Acrylamide, and length of separation in the gel should be...
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[RNA] EXTRACTION AND PURIFICATION OF TOTAL RNA USING TRIzol 日期：2005-07-18 00:00:00 点击：352 好评：0
Friday, October 17, 2003 Description RNA Extraction using Trizol reagent Procedure 1. Homogenization for Cell Suspensions a. Place 1 ml aliquots of the cell suspension in sterile RNase free 1.5 ml microcentrifuge tubes. b. Centrifuge for 1...
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[RNA] Total cellular RNA 日期：2005-07-18 00:00:00 点击：341 好评：0
Friday, October 17, 2003 Description Isolation of total cellular RNA using 4M guanidinium isothiocyanate lysis buffer and caesium chloride ultracentrifugation Large scale isolation of total cellular RNA from adult organs Whole organs are h...
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[RNA] RNA from tissues. 日期：2005-07-18 00:00:00 点击：251 好评：0
Friday, October 17, 2003 Description Procedure for extraction of RNA from tissues. Procedure 1.Place RNAlater stabilized tissue is to be used, in DEPC treated glass petridish for cutting, and cut it. Place it into a suitably sized vessel f...