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[Cell Culture] Differentiation of 3T3-L1 fibroblasts to adipocytes 日期：2006-07-24 14:27:29 点击：386 好评：2
Tuesday, June 14, 2005 Description We maintain the stock of 3T3-L1 cells in 150 mm tissue culture dishes in DMEM (Gibco catalog no. 11995-065) supplemented with 10% Fetal Bovine Serum, 100 units/ml of Penicillin and 100 g/mL of Streptomyci...
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[Cell Culture] Dorsal Root Ganglion Neuron Culture from Adult Rats 日期：2006-07-24 14:27:29 点击：376 好评：0
Sunday, June 12, 2005 Description Procedure for culturing and maintaining Dorsal Root Ganglion (DRG) neurons from adult rats. Procedure Preparing Culture Surface -Collagen coat cover slips (if using) or cell culture dishes: evenly spread 2...
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[Cell Culture] Primary neuron viability 日期：2006-07-24 14:27:28 点击：317 好评：0
Wednesday, June 01, 2005 Description Fluorescence based asssay for establishing neuronal viability in cell culture monolayers. Procedure Monolayer cultures of rat embryonic cortical neurons were prepared and plated at a density of 2-5 x 10...
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[Cell Culture] Protein extraction for SDS-PAGE from human monocytes 日期：2006-07-24 14:27:28 点击：318 好评：0
Wednesday, May 18, 2005 Description Protocol for rapid extraction of undegraded proteins from cultured human monocytes for the purpose of immunoblotting Procedure Isolate PBMCs from Buffy Coats as usual, and select CD14+ monocytes using an...
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[Cell Culture] Cell Clonogenic Survival Assay 日期：2006-07-24 14:27:27 点击：350 好评：0
Wednesday, May 04, 2005 Description Allows one to test the capability of adherent cells to survive and replicate following insult with chemicals or radiation. Procedure 1. Grow the cells in regular media to 70% confluence and treat the cel...
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[Cell Culture] Bulk synthetic RNA preparation 日期：2006-07-24 14:27:27 点击：239 好评：0
Saturday, April 30, 2005 Description A method to prepare large ammounts or synthetic RNA using T7, T3 or SP6 RNA polymerases. Procedure Template Preparation and RNA Transcription PCR generated templates or 2 x Cesium Cl2 banded plasmid DNA...
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[Cell Culture] How to freeze mamalian cells 日期：2006-07-24 14:27:26 点击：308 好评：0
Tuesday, June 29, 2004 Description This protocol describes how to freeze tissue culture cells (mamalian) in a way that they will be viable when thawed. Procedure 1.) Aspirate media 2.) Wash cells with PBS 3.) Apply trypsin/EDTA (1ml for 15...
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[Cell Culture] Cloning cells by limiting dilution 日期：2006-07-24 14:27:26 点击：333 好评：0
Monday, June 07, 2004 Description This protocol describes a method that can be used to clone individual cells. This is done by limiting dilution and examination of plates for single cells. Growth factors or selection components may be adde...
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[Cell Culture] PAH XmnI Polymorphism 日期：2006-07-24 14:27:24 点击：247 好评：0
Saturday, May 15, 2004 Description PAH XmnI Polymorphism Procedure XmnI; LOCATED ABOUT 2 KB 3' OF THE MSPI POLYMORPHISM IN INTRON 8 Template: Genomic DNA 100-200 ng/reaction volume of 25 l Primer 1. PAHXMN1 5 -CTG TAC TTG TAA GAT GCA GC-3'...
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[Cell Culture] APOB XbaI Polymorphism 日期：2006-07-24 14:27:24 点击：226 好评：0
Saturday, May 15, 2004 Description APOB XbaI Polymorphism Procedure Xba I Template: Genomic DNA 100-200 ng/reaction volume of 25 l Primer 1. APOBXB1: 5'-AAA TAA CCT TAA TCA TCA ATT GGT 3' 100ng/reaction Primer 2. APOBXB2: 5'-GGT TCT TAG CA...
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