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Thursday, November 13, 2003
Description In vitro growth of seedlings Procedure sterilisation of seeds: rinse with 70% EtOH for 30 sec put in 1% bleach (sodium hypochlorite, supplemented by a few drops of Tween-20) for 5 min wash 2-4x with sterile H2O for 1 min plating and growth resuspend in sterile 0.1% agarose plate 4 ml in petridish (0.8% agarose supplemented with 1/2 MS10) in sterile hood let dry for 1-2 h in the hood wrap with 3M micropore tape keep at 4癈 for 4 days place the dishes under light Recipes Solutions 1/2 MS10 MS Macro I 50.0 ml MS Macro II 50.0 ml Myo 10.0 ml FeNaEDTA 10.0 ml MS Vitamines 1.0 ml MS Micronutrients 0.5 ml Sucrose 10.0 g H2O x.x ml Total 1000.0 ml adjust pH to 5.7 with 1 M KOH, alternatively MES (0.5g/l) can be used to ensure more stable pH during plant growth. Supplies Tips (责任编辑：泉水) |
In vitro growth of seedlings
时间:2006-07-24 14:11来源:Scienceboard.net 作者:admin 点击:
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