Elution of DNA fragments from agarose

Sunday, June 27, 2004

Description
A rapid and very simple method to purify DNA fragments from agarose gel with yield and quality sufficient enough for routine molecular biology work.

Procedure
1. Place excised DNA-containing agarose gel slice in a 1.5 ml microcentrifuge tube and freeze at -70degC for at least 15 minutes, or until frozen. It is possible to pause at this stage in the elution procedure and leave the gel slice frozen at -70degC.

2. Melt the slice by incubating the tube at 65degC.

3. Add one-volume of TE-saturated phenol, vortex for 30 seconds, and freeze the sample at -70degC for 15 minutes.

4. Thaw the sample, and centrifuge in a microcentrifuge at 12,000 rpm for 5 minutes at room temperature to separate the phases. The aqueous phase then is removed to a clean tube, extracted twice with equal volume ether, ethanol precipitated, and the DNA pellet is rinsed and dried.

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Elution of DNA fragments from agarose