Goldmans Iron Hematoxylin

Monday, November 24, 2003

Description
Staining protocol

Procedure
Bring sections to water, removing mercury pigment if necessary.
2. Place into solution A for 30 minutes - 24 hours.
3. Wash in running tap water for 10 minutes.
4. Place in solution B for 3 hours or longer.
5. Wash in running tap water for 15 minutes.
6. Place in solution C for 1 hour.
7. Wash in running tap water for 15 minutes.
8. Blue with solution D.
9. Counterstain if desired.

Expected results
Nuclei - black
Basophil cytoplasm - grey

Recipes
Solution A
Ferric ammonium sulphate 4 g
Water 100 mL
Glacial acetic acid 1 mL
Sulphuric acid 0.12 mL

Solution B
Picric acid (sat. aqu.) 100 mL
Sulphuric acid 0.1 mL

Solution C
Hematoxylin 0.5 g
Water 100 mL

Solution D
70% ethanol 100 mL
Lithium carbonate (sat. Aqu.) 5 drops

Compounding procedure
Make each solution seperately.
The aqueous hematoxylin should be ripened before use.

Fixation and sections
Paraffin sections of material fixed with Formalin variants, Bouin or Zenker.

Supplies

Tips

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Goldmans Iron Hematoxylin