Oxidase (Spot Test Dropper)

Monday, November 24, 2003

Description
This test determines whether an isolate produces oxidase enzymes and is used for the identification of Neisseria species isolated from primary plates.

Procedure
Hold the dropper upright and squeeze gently to crush the glass ampule inside the dispenser.

Add 2-3 drops directly to the colonies to be tested and observe for 30 seconds.

Interpretation

Positive: Development of a purple colour within 30 seconds

Negative: No colour change

Note: Colonies which are positive must be subcultured immediately since prolonged exposure will result in death of the organisms.

Quality Control

Test daily with known positive and negative controls.

Positive: P. aeruginosa (ATCC 27853)

Negative: E. coli (ATCC 25922)

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Oxidase (Spot Test Dropper)