APE v2.0.46_生物行

Runs in Windows (XP, Vista and 7) and Mac (OS X v10.5 and above)
Highlights restriction sites in the editing window
Accurately reflects Dam/Dcm blocking of enzyme sites
Highlights text using pre-defined and custom feature libraries
Shows translation, Tm, %GC, ORF of selected DNA in real-time
Reads DNA Strider, Fasta, Genbank and EMBL files
Saves files as DNA Strider-compatible or Genbank file format
Highlights and draws graphic maps using feature annotations from genbank and embl files
Directly BLASTs selected sequence at NCBI or wormbase

Reads ABI sequencing trace files
Sequences in ABI traces can be aligned directly to a reference sequence, with the alignment hyperlinked back to te trace.

Selects sites matching multiple criteria (union/intersection- cut frequency, site type) in all open windows
Selects sites that cut more often in one sequence than another (for snip-SNP detection or diagnostic digests)
Has user defined enzyme grouping to distiguish eg. enzymes currently in stock.
Allows users to define new enzymes by name and recognition site
Imports DNA Strider format files (simple enzyme, site lists) available from REBASE

Other Features:

Most analysis windows are hyperlinked to their corresponding sequences, including:
- Graphic Maps
- Text maps
- Virtual Digests
- Alignments (including ABI sequences)
- Silent Sites
- Translation
- Primer Find
Uses custom feature definition libraries, which allow:
- Quick annotation of sequence
- Quick searching and highlighting of all available primers that you (or others) have that hybridize to a sequence
- Sequence to be annotated and visualized in multiple ways quickly and efficiently
- Graphic maps that show primer binding sites and all interesting sequence features
Translates sequences with optional DNA alignment
Finds potential primers matching user criteria (length, Tm, %GC, self/other complementarity)
Aligns two DNA sequences (or any combination of sequence and ABI trace), with the alignment hyperlinked to the original sequence
Finds translationally silent restriction sites
Draws graphic ORF maps