Sporulation Protocol
时间:2005-07-18 00:00 来源:Scienceboard.net 作者:admin 点击:次
| Tuesday, November 18, 2003
Description One of the suggested protocol Procedure Grow up a small (5mL) overnight culture in YEPD (rich glucose media), 30C. Count the cells the next day, and dilute the culture down to 1E6 cells/mL, again into a total volume of 5 mL of rich media. Let the new culture grow at 30 deg C until it reaches the end of log phase, which is 8-9E7 cells/mL. Spin down the cells and wash them once with sterile water. Resuspend the cells in sporulation media (2% potassium acetate, pH 7.0) supplemented with necessary nutrients. Set up this culture so that the cells are at a concentration of 5E7 cells/ mL, and a total volume of 2.5 mL. Let the cultures sporulate 5-7 days at 23 deg C. Recipes Supplies Tips (责任编辑：泉水) |