Sporulation Protocol 2
时间:2005-07-18 00:00 来源:Scienceboard.net 作者:admin 点击:次
| Tuesday, November 18, 2003
Description A different Protocol Procedure Grow cells overnight in YEPD. Remove 200 l, add to 5 ml sterile water. Spin 5 min. Resuspend pellet in 2.5 ml SPM. Incubate at 23C with good aeration for 2-4+ days and check under microscope for the formation of tetrads. Recipes zymolyase solution 0.5 mg/ml in 1M sorbitol: 5 mg zymolyase 10 ml 1M sorbitol SPM 3 g potassium acetate 0.2 g raffinose Mix in 1 L of sterile water Supplies Tips (责任编辑：泉水) |