Goldmans Iron Hematoxylin
时间:2005-07-18 00:00 来源:Scienceboard.net 作者:admin 点击:次
| Monday, November 24, 2003
Description Staining protocol Procedure Bring sections to water, removing mercury pigment if necessary. 2. Place into solution A for 30 minutes - 24 hours. 3. Wash in running tap water for 10 minutes. 4. Place in solution B for 3 hours or longer. 5. Wash in running tap water for 15 minutes. 6. Place in solution C for 1 hour. 7. Wash in running tap water for 15 minutes. 8. Blue with solution D. 9. Counterstain if desired. Expected results Nuclei - black Basophil cytoplasm - grey Recipes Solution A Ferric ammonium sulphate 4 g Water 100 mL Glacial acetic acid 1 mL Sulphuric acid 0.12 mL Solution B Picric acid (sat. aqu.) 100 mL Sulphuric acid 0.1 mL Solution C Hematoxylin 0.5 g Water 100 mL Solution D 70% ethanol 100 mL Lithium carbonate (sat. Aqu.) 5 drops Compounding procedure Make each solution seperately. The aqueous hematoxylin should be ripened before use. Fixation and sections Paraffin sections of material fixed with Formalin variants, Bouin or Zenker. Supplies Tips (责任编辑：泉水) |