Urea Slant
时间:2005-07-18 00:00 来源:Scienceboard.net 作者:admin 点击:次
| Monday, November 24, 2003
Description To determine the ability of an organism to split urea by the action of the enzyme urease forming two molecules of ammonia with resulting alkalinity. Procedure From one isolated colony, heavily inoculate the urea slant. Incubate O2, 35C. Read at 3 hours and again at 18-24 hours. Interpretation Positive: Intense pink-red colour. Rapidly positive: 1 to 6 hours (Proteus spp.) Delayed positive: > 18 hours Negative: No colour change Precautions Urea test media rely on the demonstration of alkalinity, thus are not specific for urease. The utilization of peptones or other proteins may cause an increase in pH. Quality Control Controls should be set up weekly: P. mirabilis (ATCC 12453): Positive - 4 hours K. pneumoniae (ATCC 13883): Weak positive - 18 hours E.coli (ATCC 25922): Negative Recipes Urea Slant Bacteriology loop Supplies Tips (责任编辑：泉水) |