RNA denaturing gel - BTPE
时间:2005-07-18 00:00 来源:Scienceboard.net 作者:admin 点击:次
| Tuesday, June 29, 2004
Description This protocol can be used to visualize denatured RNA Procedure Combine 1ug RNA in 10ul glyoxal reaction mix. Incubate 55 degC for 1 hr. Run out on a 1% agarose gel (made with 1x BTPE buffer) at 5V/cm. Recipes 10x BTPE: 3 g PIPES 6 g Bis-Tris 2ml .5M EDTA 90 ml DEPC H2O Then top off to 100 ml. Glyoxal reaction mix: 6ml DMSO 2ml 6M glyoxal 1.2 ml 10X BTPE 0.6 ml 80% glycerol 0.2 ml 10 mg/ml EtBr Store at -80 deg C in dark Supplies Tips (责任编辑：泉水) |