Electron Microscopy Protocols
Credits
Part A: Tissue Preparation
LTP Physiology Protocol
Vibroslicer VT 1000S
Slice Dissection and Vibratoming in Agarose
Stoelting Tissue Chopper
Microwave Processing Protocol
Schedules:
a. Standard Epon
b. Epon/Spurrs
c. Standard Epon with enhanced ribosomal
staining
Routine Embedding of Tissue
Re-embedding Tissue
Tissue Embedding for Photography
Tissue Preparation Safety Protocols
a. Protocol for Neutralizing the Aldehydes in our
Fixative Waste
b. Protocol for Disposing of Osmium Tetroxide
Contaminated Plastic Containers (solid osmium
tetroxide contaminated waste)
Part B: Microtomy
- Glass Knife Making
- Coating Grids
- Denton Vacuum Carbon Evaporator
- Photography of Epon Blocks using the Nikon Light Microscope
- Hand Trimming Epon Blocks
- Thick Sectioning
- Thin Sectioning
- Monitoring for Radioactivity
- Staining Grids
- Uranyl Acetate Recipe
- Lead Citrate Recipe
- Serial Sectioning
- Ridge Pyramid Trimming for Serial Sectioning
- Loading Serial Sections into Gimbols or Hex Rings
Part C: Electron Microscopy
- Loading/Unloading Hex Rings into Gatan Rotational Holder
- Loading/Unloading Gatan Rotational Holder into JEOL 2010
- Loading/Unloading Gimbols into Rotational Holder
- Loading/Unloading Rotational Holder into JEOL 1200 EX
- Alignment Protocol for JEOL 1230
- Photographing across the Depth of a Thin Section
- Photographing Serial Sections
- Unloading/Loading Film into the Electron Microscope
- Developing Electron Microscope Film
- Routine Scanning of EM Negatives Using Microtek Artix Scan 1800f Scanner
- Scanning EM Negatives Using Polaroid SprintScan 45
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