|
Monday, May 03, 2004
Description Culture of Dissociated Cortical Neurons Procedure 1. Use 7 to 8 day chicken embryos 2. Dissect forebrain in Ham's F12 media, peel off all meninges and then separate cortex from basal ganglia 3. Dissociate neurons by incubating with 0.5% trypsin for 20 minutes at 37C 4. Wash tissue with fetal calf serum for 5 minutes to end trypsinization 5. Dissociate tissue by gentle trituration with a flamed pipet 6. Wash 2x in F12 medium, transfer to Neurobasal Medium (NBM, Gibco) 7. Plate onto pretreated (poly-dl-ornithine or poly-l-lysine) culture dishes containing NBM or F12+ medium. 8. Culture for 2 to 3 days Recipes Supplies Tips (责任编辑：泉水) |
Culture of Dissociated Cortical Neurons
时间:2006-07-24 14:26来源:Scienceboard.net 作者:admin
顶一下
(0)
0%
踩一下
(0)
0%
------分隔线----------------------------
- 发表评论
-
- 最新评论 进入详细评论页>>
- 特别推荐
-
- 推荐内容
-