BatchFrecycled181.238.13

                    Fed-batchGrecycled45.32.275.96

                    Fed-batchG/Srecycled45.43.48.9

                    Fed-batchG/Srecycled92.64.786.14

                    Fed-batchS/Sfresh34.23.7513.3

                    BatchS/Sfresh171.288.4



          

        相关专利：WO9635799、US5599689  

          

        （三）利用酵母菌S.cerevisiae和细菌K.pneumoniae两步法发酵生产1，3-丙二醇

        第一步，利用酵母菌S.cerevisiae将葡萄糖转化为甘油；第二步，利用细菌K.pneumoniae将甘油转化为1，3-丙二醇。

        不同的菌株组合两步法发酵生产1，3-丙二醇的得率：

                    第一步第二步yieldcyieldd

                    Aerobic,46hr,30℃

                    S.cerevisiae  ATCC4132Aerobic,22hr,30℃

                    K.pneumoniae  ATCC259553.03.6

                    Aerobic,38hr,30℃

                    S.cerevisiae  ATCC64236Aerobic,23hr,30℃

                    K.pneumoniae  ATCC259554.124.9

                    Aerobic,46hr

                    S.cerevisiae  ATCC64236Aerobic,22hr

                    K.pneumoniae  ATCC259551.882.24

                    Aerobic,38hr

                    S.cerevisiae  ATCC64236Aerobic,23hr

                    K.pneumoniae  ATCC259554.174.9

                    Aerobic,46hr

                    S.cerevisiae  ATCC4132Aerobic,32hr

                    K.pneumoniae  ATCC259556.748.0

                    Aerobic,38hr

                    S.cerevisiae  ATCC4132Aerobic,32hr

                    K.pneumoniae  ATCC259553.13.7



        c:消耗100克碳水化合物产生的1，3-丙二醇的克数

        d:丙二醇碳的摩尔数/消耗碳水化合物的碳摩尔数

        相关专利：WO9635799、US5599689  

          

        （四）转甘油—3—磷酸脱氢酶或（和）甘油—3—磷酸酶基因的工程菌