Friday, October 24, 2003 Description Exact preparation of plates with lawns of bacteria for the in vitro growth of entomopahtogenic nematodes belong to Heterorhabditis and Steinernema Procedure Here's the protocol for raising nematodes on...

Sunday, October 19, 2003 Description Gel Shift with Glutaraldehyde Procedure 1. Pour a 4% acrylamide high ionic strength Tris/Glycine gel that is 1.5 mm thick with a 12 to 14 tooth comb. Combine: 6.7 ml 30 % Acrylamide (60:1), 10 ml 5X Tri...

Sunday, October 19, 2003 Description Labeled nucleotides from the RNA are covalently transferred to bound proteins following illumination by UV light. Excess RNA is then digested with Ribonuclease A and the labeled proteins are visualized...

Saturday, October 18, 2003 Description TNF-alpha Bioassay Procedure 1) Prepare L929 cell suspension at a density of 3.5 x 105/ml in assay medium. Add 100 l/well to the 96-well Assay Plate and incubate overnight at 37 degrees C, 5% CO2 in a...

Saturday, October 18, 2003 Description Flow assays allow visualization of cell adhesion under well-defined wall shear stress. The visualization of the different events of cell adhesion can be quantified by selective image acquisition and s...

Saturday, October 18, 2003 Description This method uses carboxyfluorescein succinimidyl ester (CFSE) rather than fluorescein isothiocyanate, resulting in more reliable labeling. Succinimidyl esters are excellent reagents for amine modifica...

Thursday, October 16, 2003 Description TRANSFECTION OF MAMMALIAN CELLS USING LIPOFECTAMINE REAGENT Procedure 1. In a six-well or 35 mm tissue culture plate, seed ~2x 105 cells per well in 2 ml Basal Medium containing 10% FBS. 2. Incubate t...

Wednesday, October 01, 2003 Description Alkaline phosphatases (APs) are highly ubiquitous enzymes, present in all species from bacteria to man Procedure 1.Grow bacteria overnight in Pi sufficient and Pi deficient media. 2.Read OD600 of ove...