Monday, November 24, 2003 Description To determine the ability of an organism to split urea by the action of the enzyme urease forming two molecules of ammonia with resulting alkalinity. Procedure From one isolated colony, heavily inoculat...

Monday, November 24, 2003 Description This test determines whether an isolate produces oxidase enzymes and is used for the identification of Neisseria species isolated from primary plates. Procedure Hold the dropper upright and squeeze gen...

Monday, November 24, 2003 Description Staining protocol Procedure Bring sections to water, removing mercury pigment if necessary. 2. Place into solution A for 30 minutes - 24 hours. 3. Wash in running tap water for 10 minutes. 4. Place in...

Monday, November 24, 2003 Description Staining protocol Procedure Bring sections to water via xylene and ethanol. Do a Mallory bleach. Wash with running tap water Oxidise in solution A for 10-20 minutes. Rinse well with water. Place into s...

Friday, November 21, 2003 Description The best known and distinctive property of the genus, Mycobacteria, depends upon their lipid-rich cell walls which are relatively impermeable to various basic dyes unless the dyes are combined with phe...

Friday, November 21, 2003 Description The best known and distinctive property of the genus, Mycobacteria, depends upon their lipid-rich cell walls which are relatively impermeable to various basic dyes unless the dyes are combined with phe...

Friday, November 21, 2003 Description The preparation of a smear is required for many laboratory procedures, including the Gram-stain. The purpose of making a smear is to fix the bacteria onto the slide and to prevent the sample from being...

Friday, November 21, 2003 Description Gram-staining is a four part procedure which uses certain dyes to make a bacterial cell stand out against against its background. The specimen should be mounted and fixed on a slide before you procede...

Friday, November 21, 2003 Description Agglutination Assay Procedure Passive agglutination is performed using tanned sheep red blood cells and P. aeruginosa LPS (heated for 1 hour at 100oC prior to use). Sheep erythrocytes are washed three...

Friday, November 21, 2003 Description Agglutination Assay Procedure Bacterial agglutination is performed using either P. aeruginosa cells autoclaved at 120oC for 1 hour, then centrifuged and resuspended in saline or live P. aeruginosa cell...